...(Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16, 2014, that... it has been repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing...specifies the target, the Cas9 endonuclease that creates the DNA double-strand break (DSB) at the target...mouse zygote (Qin et al., 2015).  When inside the zygotes, the gRNA will seek out its target among the 3 ...

...the surface of extracellular pathogens such as parasites or microbes, or to proteins expressed on the surface...cascades that clear these infections. Anything that generates an antibody response in the immune system is referred...at different times of the immune response and initiates different immune cascades. In the research setting...quantitative measurement of protein expression in cell lysates, whole cells, or tissue samples. Many experiments...

... announce the winners of our 2021 Deck the Lab Contest! Though 2021 had more than its fair share of challenges... challenges, the annual Deck the Lab Contest brought us smiles and joy. In fact, our Addgenies were absolutely...festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https...

...through 2009, though I suspect that our costume contests were indeed occurring earlier but that the photo...protect the innocent revelers. Our Halloween costume contests have become an annual tradition in which we choose...finding out who wins this year’s Addgene costume contest, like us on Facebook and checkout our FB page on...

...only March!)  In our repository, you'll find the latest iteration of GCaMP, GCaMP8, available in new serotypes...repository  Name Serotype Category Depositor  Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New...forgot to use the link below to keep up with the latest in Addgene's viral vectors services and resources...

... develop tests for food products as opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens...how It works 18:02 - 19:56 : Why Sample 6's first test was for Lysteria 19:56 - 24:41 : Who is using Sample...

...you need access to raw data, the target sites, off-target sites, scores and the statistics that go into...genome contains only 0.66 million targetable NGG PAM sites whereas the human genome has about 300 million. ...newer tools may still prove somewhat useful for the latest NTT targeting Cas protein, Cpf1. Separately, on-target...

...environmental impact of those processes? Who even regulates them? You may be surprised to learn that it hasn... been 50 years since the creation of the United States Environmental Protection Agency, which started ...scientists washed and reused materials such as glass pipettes or inoculation loops. While single-use is now ...

...So, as 2013 comes to a close, let’s take a few minutes to reflect on some of the most noteworthy developments...database to gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly from...granted via a public repository or authors’ own web sites. 9)      The year 2013 has also been the year of...

... was pumpkin. Everyone gets to vote for their favorites, and trophies are awarded and coveted. I’ve only...we head into Thanksgiving weekend in the United States, I look forward to gathering around a large table...you have any traditions that you share with your labmates, be they food related or not? We would love to...

...to activate gene transcription. The researchers tested a variety of combinations to optimize both fusion...500nm). By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft of Cas9, the fluorescent...Upon light activation, dCas9 is released within minutes and red foci formed 20-40 min later. The CASANOVA...synthetase, read this article). The group first tested photocaging various lysines in Cas9 to determine...

..., M.W., Nguyen, T.H., Coxe, M.A., Miller, B.C., Yates, K.B., Gillis, J.E., Sen, D.R., Gaudiano, E.F., ..., Haining, W.N., & Sharpe, A.H. (2019). PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations...0480-4 LaFleur, M. W., Nguyen, T. H., Coxe, M. A., Yates, K. B., Trombley, J. D., Weiss, S. A., Brown, F....

...months of strategizing, designing, developing, testing - and repeat - we’re proud and excited to finally...homepage While users are increasingly accessing websites other than through the ‘front door,’ the homepage...Additionally we also wanted to highlight our blog, daily updates, and offer better exploration throughout the site...

...transcriptional regulators. In this system, Cas9 generates a double-stranded DNA break at the genomic loci... the universal sgRNA is not predicted to target sites in the human, pig, or zebrafish genome, this system... Using the structure of CasX at eight different states of assembly from cryo-electron microscopy, the ...

...to make it more accessible. Other accessibility updates include a printer-friendly design and alt text ...on all images. We have also made accessibility updates to our website, which receives 3.8 million visitors...perspective on Addgene through her LinkedIn page. She invites you to connect with her there. More on the Addgene...

...for scientific titles generated from the United States Standard Occupational Classification System  and...female to male scientific authorship in the United States (0.428). 4. The #1 (re)tweeted domain by scientists...scientists was Instagram Social media and news sites were also popular. Perhaps scientists, like nonscientists...

...bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids...segment of DNA which contains several restriction sites allowing for the easy insertion of DNA. In expression...bacterial cells and selectively grown on antibiotic plates.  Importantly, because the bacteria from which ...

...physical and chemical conditions like YNP thermal sites - are also of practical interest since they’ve previously...distance. At each collection site, we took GPS coordinates, water temperature readings using a heat gun,...the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9 is...

...used in optogenetic studies are not native to vertebrates and are commonly introduced in zebrafish through... a neural circuit involved in locomotion that generates periodic motor commands for rhythmic movements...  Find plasmids from the Isacoff lab! They then tested what kind of swimming behavior, spontaneous swim...

... organs located deep in the body, like the gastrointestinal tract. Require Administration of Substrate...coli aided in ultrasound imaging of the mouse gastrointestinal tract. The E. coli strain Nissle 197 (ECN)...probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years to clinically...