Plasmids 101: Yeast Vectors
Type
Blog Post
Published
Feb. 25, 2014, 7:11 p.m.
... the 2 micron circle (a natural yeast plasmid) allows for 50+ copies to stably propogate per cell. The...within an E. coli cell, but vectors can be widely utilized across many different cell types and each one... the yeast cell. These elements control not only the number of plasmids found in each cell, but also whether...are eukaryotes and thus contain complex internal cell structures similar to those of plants and animals...components to allow shuttling between E. coli and yeast cells. To be useful in the lab, the vectors must contain...replication (ORI) and a means of selection in yeast cells, in addition to the bacterial ORI and antibiotic...determines plasmid copy number within the bacterial cell. Similary, the specific ORI elements included within...