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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 798 results for: OMP;

Showing: 361 - 380 of 798 results

  1. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...degradation In the Bradner and Gray labs, we synthesize compounds that enable selective removal of proteins-of-interest...FKBP12F36V-tagged proteins. The dTAG molecule induces ternary complex formation between the FKBP12F36V-fusion and the... for degrading targets in different cellular compartments, and in vivo utility. dTAG molecules will be...lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal fusions and...

  2. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...is dependent on proteasome function. TRIM-away compared favorably to drug inhibition, with TRIM-away targeting...larger cell populations, you can deliver these components together using electroporation. In these bulk...250 nm), and studying endocytosis is further complicated by the reliance on pH-based biosensors used to...rapid and reversible recruitment of chromatin complexes to any genomic locus in any cell type. By varying...

  3. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...process that’s difficult for individual labs to complete. The nanobody based RANbody platform from the ...the sample due to the smaller size of RANbodies compared to antibodies. Staining with multiple HRP-RANbodies...sample is stripped. HRP-RANbodies are, however, compatible with most multi-labeling immunohistochemical ...antigen with a primary/ labeled secondary antibody. Compatible with multi-labeling protocols when other antigens...

  4. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...show. After graduating with a master’s, Susan completed a science writing internship with the National...planning.” To beef up her resume, she decided to complete a certificate in Strategic Communications and ...the most satisfying parts of the job is having completed one of these difficult pieces: “It’s probably ...science communication] needs. I worked for one company, but there were a ton of others that also did work...

  5. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... are considering to make sure that they’ll be compatible with your instruments, each other, and your target...target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores. We also touched on fluorophores... sure to check that your antibody’s buffer is compatible with your conjugation kit, since many common ...reactions. If needed, you can often remove the incompatible agents by dialysis or gel filtration.    There...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...recombination events. The system consists of two components derived from the P1 bacteriophage: the Cre recombinase...recognition site. The P1 bacteriophage uses these components as part of its natural viral life-cycle, and ...and researchers have adapted the components for use in genome manipulation. Cre recombinase, originally...other sites of the same type, but are not cross-compatible. Using different lox site variants allows Cre...

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... is important to fully characterize the compound, or compounds, you choose to ensure reliable results ...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...pharmacodynamic actions of clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific...

  8. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...’ll share the basic composition of these solutions below. Alternatively, companies including ThermoFisher... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...lost each time you strip a blot, you should not compare the strength of bands from different rounds of ...

  9. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted expression was...retina.  Find the AAV synthetic promoter plasmids! Comparing synthetic promoters performance in mouse, monkey...targeted cells in monkey and human retinas. By comparing the performance of promoters across these three..., Szabó, A., Gross-Scherf, B., Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...optogenetic or chemogenetic receptors) enables complex control of specifically defined neuronal populations...how newly generated neurons integrate into the complex pre-existing circuit of the brain. Another study...anatomical regions of the brain receive inputs and compute outputs. In general, RABV monosynaptic tracing ...circuitry, and continue to contribute answers to the complex questions of how the brain operates. References...

  11. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... formation of replication competent lentivirus (RCL). Replication competence can arise from recombination...drive high levels of expression of certain viral components; according the manufacturer these changes result...downstream. When assessing lentiviral titer, don't compare apples to oranges After choosing a packaging system...

  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...up clear tasks for each member of the team to accomplish so that many elements of the project were able...involved definitely contributed to our ability to complete the project on such a short timescale. Our article...extensive infrastructure of molecular and electronic companion technologies that has transformed the assay’s ...of Zika Virus Using Programmable Biomolecular Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids...

  13. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...better at producing a compound of interest, thus allowing them to optimize the compound production process...fluorescent proteins have usurped old methods or complemented them in ways that make them much easier. A special...Although it is possible to make a timer system composed of two separate FPs (one that matures quickly ...

  14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...protein capsid encapsulating the genome is majorly composed of three proteins that can be easily modified ...Delivering gRNA and Cas9 using adenoviral vectors As compared to other viral vectors, AdVs can provide a large...AdV-mediated transduction of gRNA:Cas9 ribonucleoprotein complexes into transformed and non-transformed cells yielded...vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array...

  15. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... capture large field-of-views (6). For more comprehensive reviews on several other LSFM configurations...multiplying couple-charged device (EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it...labeling in whole tissue (15).  CLARITY is also compatible with LSFM, a method termed COLM (clarity-optimized...spatiotemporal control over labeling methods as compared to transgenic animals (17).  These viral plasmids...

  16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...For example, Drosophila lines with compound autosomes are completely infertile when mated to wild fruit...with appropriate measures. Barriers should be a component of all gene drive confinement strategies, but ...: I. Cage Studies of Chromosome Replacement by Compound Autosomes in Drosophila melanogaster. Genetics...

  17. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...his program. Initially, Provost made his labs completely open-ended, allowing every student to work on...Kowalski et al., 2016; Scott, 2021). In fact, completion of Provost’s CURE increased students’ confidence...them to near homogeneity using a 6XHis tag, and compared their specific activities by measuring NADH production...Poirier, V., Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor cell maintenance...

  18. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ... an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine...off-target editing. In one direct transcriptome-wide comparison, REPAIRv2 reduced off-target sites from the 18,385...edits by ~45% and off-target A-to-I edies by ~94% compared to RESCUE.   Addressing the potential use of RESCUE... Abudayyeh, Omar O., et al. "C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector...

  19. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...orders were affected by the COVID-19 pandemic, we compared the number of plasmid orders and new COVID-19 ...the likelihood in our Poisson process into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential...effects fairly well. However, we observed one complication in the China data – there is a period of 1 month... lockdown in China, then the observed data is compatible with an infinity-fold reduction in activity due...

  20. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...real time. When the PCR is complete, the results are analyzed by comparing the threshold fluorescence ...amplification reaction to take place.  When the PCR is complete, a droplet reader extracts the droplets from the...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...
Showing: 361 - 380 of 798 results