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Showing: 361 - 380 of 645 results

  1. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

  2. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...scientist-in-training will spend 10 or more years with a small number of formal advisors learning how to be a scientist...

  3. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not...of viral vectors (which many researchers will informally refer to as viruses) are named after the viruses...

  4. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... is present in this line, making it genetically female. HEKs are generally cultured as an adherent monolayer...blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and...

  10. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...genomic drift or limited material from polyclonal animal preparations (Bradbury et al., 2015). All of Addgene...were tested for specificity in knock-out lines or animals.  Addgene will continue to add more antibodies,...

  11. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ...

  12. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...to request support for our operations until our normal work resumed. Addgene distributes and fulfills ...supporting research could provide Addgene with a small amount of funds, the community as a whole could ...

  13. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...valuable for CRISPR/Cas9 stem cell editing. The small molecule NSDB and Na+ hypertonicity are key elements... Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. ...

  14. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...handling recommendations Not all O-rings function optimally at -80°C. For samples that are shipped on dry ...changes in our solutions are transient and return to normal once the tube is opened and equilibrium with ambient...

  15. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important elements...enzymes cut at known sites within the ITR. However, small deletions and point mutations will go undetected...

  16. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs nor does it have high levels of off-target...changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from...

  17. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...and has extensive experience targeting RNA with small molecules. Other members’ projects range from hacking...Ph.D. grad student at Boston University studying mammalian immunology, and in his free time, he is pursuing...

  18. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...plasmids in the library are differently sized, smaller plasmids may be amplified preferentially, skewing...Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids in the library ...

  19. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...yourself to be as productive as you would be in a normal situation. For some other resources on working...Science, or Resources to Engage Kids at Home. It’s normal and okay to feel overwhelmed right now, but hopefully...

  20. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...examining relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed...doesn’t love a book series that features a young female protagonist, some clever magic, and a satisfying...
Showing: 361 - 380 of 645 results