Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
Type
Blog Post
Published
Aug. 27, 2020, 1:15 p.m.
...such experiments, small-molecule inhibitors are tested in parallel for their ability to prevent BiFC resulting...preferably, some information about the structures of the tested PIPs. Many split FPs have been validated for BiFC...- and C-terminal end of each PIP and empirically test which combination provides the best fluorescent ...appropriate controls may requires that you empirically test a number of mutants to identify those that inhibit...