...creates the DNA double-strand break (DSB) at the target site, and a donor oligonucleotide or plasmid as the... Find CRISPR Plasmids for use in Mammals Check out Our CRISPR Guide Pages Find Plasmids for Mouse Targeting..., the gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected...provided (in the form of an oligonucleotide or plasmid), precise changes can be made in the genome via...mice carrying the intended mutation. With a donor plasmid, the outcome is less predictable. In our best case...

... be used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies... target protein. However, small mutations in the DNA of the hybridoma can occur, known as genetic drift...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...protein (Wang et al., 2013). These proteins are also plasmid-derived. However, a disadvantage to ScFv is their...nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common internal control ... as well as tools for developing and producing plasmid-based antibodies - and we’re always growing our...

...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...meaning “to fit”. SELEX could be used to classify DNA aptamers as well, and over the course of the next...

... FRET-based biosensors, and interactions between DNA and proteins. FRET, in theory, is an easy technique...used pairs to measure FRET. The table below lists plasmids that can be used to create your choice of fluorescent... fusion protein with your gene of interest: Plasmid Color Expression Description pPROEX Aqua Cyan...

...vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target cells' genomes... ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out Our Cancer Resources Page...

...self-complementing structure (scAAV) (7). As single stranded DNA viruses, AAVs normally require second strand synthesis... promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001): 1248. PubMed...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

... M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability detection. Cell. Mol. Life...Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...

...transgenic flox animals to introduce modifications at the DNA level in a temporally and locally defined manner.... share ways how scientists have used Addgene’s plasmid repository and viral service for their behavioral...

...essential-but-unlauded role in the identification of DNA’s double helix. Perhaps to the dismay of middle-aged...and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As ...fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up in this...

...currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member of the Quality...Quality Control team, I help make sure the plasmids that go out our doors are correct. Now that I am heading...

...knockout and expression construct on the same piece of DNA. We do a lot of protein-protein interaction work ...Quick, Versatile Plant Transgenesis with GreenGate Plasmids  ...

...use the chromatin IP (ChIP) technique to identify DNA binding proteins involved in histone modification...antibody posts  Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene ...

...expensive) synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly... Generate Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent...

... non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the...on Addgene.org Check out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries...fail to account for the effects of Cas9-induced dsDNA breaks. Are off-target cutting patterns in genome-wide...function, i.e. sites that lack open chromatin marks, DNase hypersensitivity, or are in an enhancer, transcription...control for the effects of guide expression and dsDNA breaks. Figure 2: Non-targeting vs. safe-targeting...for the inherent effects, or background noise, of dsDNA breaks caused by CRISPR. See supplemental figure...

...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...developed a self-labeling fusion tag based on the human DNA repair protein AGT that gets covalently linked to...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...

... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...neuronal cells. The AAV genome is a single-stranded DNA molecule and AAV vectors are replication deficient...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...than any other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...

...relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression constructs...Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...

...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ... 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity ...2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP... Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable...novel RNA targeting system by: Susanna Stroik The Doudna lab’s recent work characterized a new tool for ...

...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...specific compartment. If your proteins are nuclear- or DNA-binding proteins, you may need to sonicate your lysates...

...packaging plasmids. The cells need three different plasmids to produce AAV: 1) the RepCap plasmid which provides...going to lie, AAV transfer plasmids are kind of a pain to work with. These plasmids contain ITRs which are... AAV transfer plasmids. It’s easy to contaminate a workspace with plasmid, not that I would know about...prep. AAV samples are first DNaseI digested to remove any residual AAV plasmid that was carried over from...sample for the DNAseI digest. Use good sterile technique when handling your AAV plasmid standard and AAV...AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses adenovirus genes which help mediate...mediate AAV viral replication; and 3) the transfer plasmid which encodes a transgene of interest that’s packaged...