...transformation acceptor vectors, and sequences for genomic integration by homology directed repair. Using ...

... for your synthetic biology experiment Read our Genome Engineering Guide ...

...pathway for the ncAA integrated into the cell’s genome, instead of incorporation via plasmid or media....

...thousands of plasmids, using both mass production genome wide screens and the more traditional “one at a...

...barcodes that are integrated into target cells' genomes upon infection. When using the ClonTracer system...

...receptors and signaling cascades conceivably allows the genome to sense the impact of the total environment on...

...in years past, we've been working with the Rare Genomics Institute to provide plasmid grants to researchers...

...reversible recruitment of chromatin complexes to any genomic locus in any cell type. By varying which sgRNAs... FIRE-Cas9 for inducible interrogation of the epigenome Article contributed by Joanne Kamens Listen to...new system, Fkbp/Frb Inducible Recruitment for Epigenome editing by Cas9 (FIRE–Cas9), combines these approaches...

...Loures, L. et al. A modular cloning toolkit for genome editing in plants. BMC Plant Biol 20, 179 (2020...

...have to be minimum around 1012-1013 vg/ml (vector genomes per milliliter) and lentiviral vectors around 108...

... can be used to integrate the tagged AID* into genomic sites of interests via CRISPR techniques. This ...

...G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https://doi.org...

...on the Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about...

... Cre. Could Cas9-DOG be developed soon to allow genome modification only in a small subset of cells? Other...

...Director of Education for the Jackson Laboratory for Genomic Medicine and a Senior Research Associate at the...

... types of DNA, such as cDNA, PCR fragments, or genomic DNA. Also remember that commercially available ...

...expression of genes integrated into the insect cell genome. Both of these types of insect expression systems...

...induction of T7 polymerase encoded in the E. coli genome (usually controlled by IPTG). For this IPTG-induced...

...carries a small, approximately 4.7 kb single stranded genome. AAV belongs to the family Parvoviridae and requires...

...questions around climate change, biodiversity, or genomes requires lots of data. Get your community involved...