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Showing: 341 - 360 of 645 results

  1. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...started with tissue culture The cultivation of mammalian cells in the lab, or tissue culture as it is commonly...called, is a critical tool for many scientists. Mammalian cells provide scientists with the means to study...

  2. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...the transition now to being general counsel of a small start-up. For the last 20 years, I was a patent ...very different jobs. Being general counsel at a small start-up is very interesting. It’s very varied. ...

  3. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...References 1. Georgieva, Maya V., et al. "Inntags: small self-structured epitopes for innocuous protein tagging... high correlation for protein localization in mammalian cells." Nature methods 10.4 (2013): 315-323. Pubmed...

  4. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...progressiva (a.k.a. FOP), that, while affecting a small number of people, deserves our attention and has...Plasmid Formats Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection...

  7. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...has been shown to work in plant, Drosophila and mammalian cells, and it may find wide use in biological ...efficiently photoconverted in plant, Drosophila, and mammalian cells. Photoconverted GFP is compatible with other...

  8. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ... is has been featured in Time magazine . Other smaller competitions are now taking place and gathering...moonlighting as artists can compete in the Nikon Small World Photomicrography Competition - a 40 year old...

  9. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... 50 base pairs of sequence homologous to the chromosomal site of insertion. The linear PCR product is ...Based on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized...

  11. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...it’s a probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years... for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed PMID: 29300010...

  13. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

  15. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1. In SunTag nomenclature throughout...Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org ...

  16. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...the same in both cases, but activation depends on small molecule activation (chemogenetics) or light (optogenetics.... TL;DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics...

  17. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...and improves overall communication.  Maintain informal communication In between recurring meetings, you...this casual communication reflects the type of informal communication you’d likely have with members of...

  18. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    .... – CRISPR can be used to generate cell line or animal models which have the precise disease genetics.... drug selectively kills the wildtype line with minimal to no toxicity in the knockout line, it is likely...

  20. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ... of interest. You can filter CLASTR results by minimal number of markers and similarity score match. This...allele search preferences to help you identify proximal lines you care about. If you have cell lines without...
Showing: 341 - 360 of 645 results