...the correct size, and purify the DNA fragments. Finally, you simply incubate these three fragments together...loop (as might be anticipated to occur within a terminator sequence), since this would directly compete ...one step with no scar sequences remaining in the final product. The long overlapping region between fragments...different fragments. One way around this is to use a combination of standard sequences with overlap regions, such...

...To acquire these measurements, the cytometer illuminates the passing cell with a laser and detects the...colleagues.   Figure 1: The laser pulse illuminates the cell and is scattered forward at a low angle...represents the time and intensity of the cell’s illumination (see Figure 2). H describes the maximum signal...For our last example plot, I will show you a combination of two stains, in this case through conjugated...

...This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed...outlined below, the technique can be used as a preliminary validation on your bulk population. This way ...TIDER (Tracking of insertions, deletions, and recombination events). TIDER can screen for specific homology-directed...

...American system), in 2009 (at the beginning of the financial recession) I knew obtaining work in scientific...facility, where I tested materials for bacterial contamination. It was a really interesting job at first, everything... are rapidly expanding, as this might not be sustainable. After working at this company for several years...would produce the best outcome for a specific combination of genetic mutations. This is a particular challenge...

...engineered proteins responsive to light in combination with retinal) variations designed to give researchers... R., Gunaydin, L. A., Hyun, M., Fenno, L. E., Gradinaru, V., Yizhar, O., & Deisseroth, K. (2012). Principles...

...then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose for...receptor binding protein hit and can go back to the original plate to identify which phage gene encodes that... 1 M NaCl in order to enhance folding of the recombinant proteins. Optimal salt concentration will vary...colony expressing a functional binding protein was originally located. Now you can grow up your colonies of...

...testing several vectors and packaging cell line combinations, Miller et al. showed that infectious wild-type...are capable of transducing certain quiescent or terminally differentiated cells such as macrophages and ...research due to the possibility that mutation or recombination with human retroviruses could lead to RCL. ...of retrovirus packaging cell lines to avoid recombination leading to helper virus production." Molecular...

...includes: Michelle, the Senior Scientist who coordinates all kit creation and quality control efforts ...once famously said “I think this is the most extraordinary collection of talent, of human knowledge, that...successfully doled out to multiple people: someone to coordinate, someone to decide, someone to delegate, etc....Next Great Discovery is exactly that: a myth. The original Dr. Frankenstein from Mary Shelley’s novel worked...

... contest is now closed. Stay tuned to see the finalists and vote for your favorite! Our mascot who has...neutral name. The Addgenies will choose up to four finalists and we’ll ask you to vote for your favorite name...We hope you submit your best ideas because the finalists will receive some newly designed Addgene swag....

...Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan The job of scientists to observe how things... and generation is in the veracity of its work. Alina Chan is a postdoctoral researcher at Harvard Medical...saying that climate change is a hoax. This has culminated in the current President holding and campaigning...interactions, and science education. Photo cred Alina Chan. Seattle, Washington, USA 47.6062°N 122.3321...

...structures are more stable and local movements predominate (e.g. a basal progenitor cell migrating to the...fluorescent proteins (Salomonnson et al. 2012). Finally, it is worthwhile to test the photostability of...used to study B and T cell dynamics within the germinal center of murine lymph nodes. However, one must...marking and tracking cells is the the Cre/lox recombination system. A researcher can flank fluorescent proteins...

...and important details that you won’t find in the final publication and we have already collected over 100...accelerate your research. Sincerely, Melina & The Addgene Team ... Melina Fan...

...Working on the Eye 9:05-10:16 : Cell Targeting in Retinal Gene Therapies 10:17-14:34 : Progress in Treating...type-specific manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015): 1334-1341. PubMed... retina...

...renewable electricity is certainly not the only sustainability effort we’ve implemented at Addgene. The kitchen...check out Leila Haery’s blog series, “Bringing Sustainable Practices to the Lab.”  Whether participating...blog Subscribe to the Addgene blog Read our sustainability in the lab blog posts Read another blog post...

...of His-tagged human kinases in bactera. Listen to Our Segment on The Human Kinase Domain Constructs Kit...Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's Lab -...

...The weather is finally warming up in Boston and that means summer is right around the corner. Here at...other video series.   And if you haven’t seen our webinar “Navigating an Expanding Landscape for Engineered...which we will be posting in the next few weeks.)  Finally, Addgenies love sunshine and warm weather - check...

...taking steps to increase plasmid distribution and disseminate knowledge around the globe. Addgene’s progress...inception in 2004. In the near future, we hope to eliminate much of white space on this map and increase plasmid...the Addgene Blog Learn About Our Recent Visit to China Read Zafira Castano Corsino’s Recent Post on the...

...project that would be a big deal if it worked out.”- Melina Fan, Chief Scientific Officer “Choose your PhD ... Tsang, Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting...customized letter to the place that you are applying.” - Melina Fan, Chief Scientific Officer If there’s a career...

...viral vector is used to deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It... work with in neurons or you might use it in combination with Flp-FRT. Additionally, you could use the... cre-recombinase...

...lab heads have revealed that a combination of hard work, determination, passion, and patience are required... time for fun and life outside of the lab? This final post in the PI Interview Series investigates how...