...Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13...

...Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic...

...packaging transfections Find tips for a first time CRISPR user Resources on Addgene.org Learn how Addgene...

...Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about...

...Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources on Addgene.org...

... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic...

...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...

...genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491. PubMed...

... Mice can be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila...

...revolution in genome editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous...

... interactions Learn how biotin can be used with CRISPR Additional resources Idtdna educational pages...

...detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for...

...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519... Gilbert Labs have previously deposited their CRISPRoff system — a programmable epigenetic editor consisting...deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins than SpyCas9...

...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...

...Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering Resources...

...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which...

...sequence cloned into it. gRNAs are used for directing CRISPR/Cas9 to the appropriate genomic location, while...

... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better...

...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...

... 96 parts including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor...promoters, and inhibited gene expression using CRISPRi. The kit functions in the model cyanobacterium ...