Optimizing Donor DNA for Enhanced CRISPR Genome Editing
Type
Blog Post
Published
March 24, 2016, 2:30 p.m.
...strand in Figure 1 below) was free to anneal to complementary ssDNA molecules in vitro.
We next wondered if...discovered that single stranded donor DNA:
Complementary to the released strand,
127bp in length,
With...target DNA that was accessible to anneal with complementary single stranded DNA. We thus wondered if such... any of the error-prone repair that normally accompanies Cas9 cutting. We still don’t know the mechanism...