Plasmids 101: 5 factors to help you choose the right cloning method
Type
Blog Post
Published
Aug. 21, 2018, 12:31 p.m.
...(the precise mechanism isn’t well understood).
Finally, TOPO cloning is another good option to consider...integration sequences, relies on site-specific recombination reactions catalyzed by BP Clonase and LR Clonase... to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The ligation-independent...since one can pool the components and define their final order through sequence homology. Similarly, Golden...