...changes in enzyme function can lead to surprisingly large fitness effects during adaptive evolution of antibiotic...

...modulate biological processes, but it did not show large changes in binding affinity with light stimulation...advantageous compared to other viral systems due to its large cargo capacity, minimal cytotoxicity, and broad ... CRISPR-Display uses the dCas9 enzyme to deploy large RNA cargos to specific DNA loci. In CRISPR-Display...

... to reduce false positive rates and control for large numbers of comparisons. The Bonferroni does this...arranging all calculated p values from smallest to largest and ranking them (1 being the smallest value). ...calculates the cut off for significance by finding the largest p value where its rank divided by the total number...

...therefore determines the timing of gene expression and largely defines the amount of recombinant protein that ...

...on many different levels - it’s hard to process large amounts of information and, from a SciComm perspective...results. As biological researchers deal with larger and larger datasets and use computer based analysis techniques...

...models remain more difficult to make. For example, large insertions, like a fluorescent protein knock-in,...

...) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly large protein, and this fusion allows for packaging into...genomic targets. These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9...

... without causing issues associated with cloning large fragments. After fragmentation, repair sheared ends...

...mechanism for sgRNA delivery and providing a means for large scale functional screens. For more information on... kinases Receptor tyrosine kinases (RTKs) are a large class of cell-surface receptors which play a critical...

...Getting a vector into a particular cell type depends largely on the viral capsid, but specificity doesn’t end...

...their environment. These types of interactions to a large extent involve receptors expressed on the cell surface...

...preferential tropism for different brain regions is largely due the ability of different serotypes to infect...is fine and a researcher should aim to inject a larger amount of vector using a serotype with strong CNS... of adeno-associated virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal...

...The best applications for dsDNA inserts include: large insertions or deletions, including selectable DNA...

...require high read counts (fast multiplexing) Requires large cell population, senstive to time since cell fixing...; Pattanayak et al. 2013). This approach misses larger mismatches (e.g. six nucleotides) that may still...

...are sensitive to distortions in helix shape (22). Large purine:purine mismatches and most smaller pyrimidine...

...useful for intracellular staining where binding of large immune complexes may be hindered. Indirect staining...information about the relative size of the cell; larger cells produce more FSC. Light that scatters sideways...

...) with 30-60 basepair homology arms to knock in large epitopes, such as GFP (Paix et al., 2014). A further...

... at the same time pass over in silence his very large one” (http://oll.libertyfund.org/titles/galilei-...that the community of science supporters is much larger and stronger that I expected. Jennifer Stancill...

...-resolution imaging, they still do not emit the large number of photons achievable with photoswitchable...

...can help reduce off-target activity. Deleting a large region of the genome by removing the sequence between...