...currently houses over 890 CRISPR screens in 629 cell lines, each manually curated and annotated from more than...and power of CRISPR-based library screens in cell lines from humans and other species. By accurately compiling...browse through screens performed in relevant cell lines or conditions. This increased ease of access not...screens performed in 26 different breast cancer cell lines. Screenshot updated 2024. Each screen curated...

...two genes: the replication (rep) gene and the capsid (cap) gene. In wild-type AAV, these two genes are...which contains the AAV structural and packaging genes, the adenoviral helper plasmid which contains the...Next let’s take a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products...

...all know that the Fc domain provides structure, determines isotypes, and provides a place for secondary ...cannot survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...induced “stabbing” and “poisoning,” but let’s be honest, the elimination style the immune system is most... that!    References and resources References Clynes RA, Towers TL, Presta LG, Ravetch JV. Inhibitory...

...Giving honest and helpful feedback gets easier with practice so you must build it into your routines. This...their work.  Check out Joanne's Reddit AMA   Guidelines for giving feedback Use formal and informal ...differently? Beware of indirect language You must be honest about performance problems. While giving corrective...

... Encodes insert(s) A plasmid with one or more genes, protein coding sequences, or other elements inserted...designed for cloning a gene/insert into it. Empty backbones often contain epitope tags or fusion proteins ...Insert *Note: This page will not appear for empty backbones Please enter the name of any gene contained within...Addgene ID in the "Backbone Manufacturer" box for backbones requested through Addgene. For example, you could...Addgene qualifies as BL1 or BL2 as defined by NIH guidelines. I hereby certify that I have disclosed all known...

...raw plasmid sequence and display frequently used genes and control elements. Development of this tool required...feature sequences due to genetic drift and the use of genes from different microbial strains. It proved to be...sequence feature that may be used to make fusion genes, detection needs to occur even if one or two codons... our database, or if you see an opportunity to harness common feature detection in a new way, please contact...

...Kamens several years ago through the Healthcare Businesswomen’s Association and it was through this connection... systematically test the function of all 20,000 genes in disease models and then focus drug discovery ...early age, I was fascinated by the impact that medicines can have on human health. As a teenager in high...the field of gene therapy.” He’s also worked on vaccines throughout that time. Now Vandenberghe is an Associate...

...issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture ...production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et al., ...hybridomas can lose gene expression of the mAb-encoding genes, die, or fail to revive after cryopreservation (...Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, ...

...capable of robust expression of high G+C content genes, as validated through monitoring the expression ...adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library...MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied for identification of essential...pooled library from Junjie Chen's lab targets human genes known or suspected to be involved in DNA damage ...

... Addgene to gene-edit mutations into XP genes and other genes involved in photosensitivity in melanocytes...hemiplegia) that are often painful and accompanied by migraines. Those attacks can be brought on by a number of...plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models carrying different SOX10 mutations...

... cell type of interest better than immortalized lines derived from the same cell type; however, their ...interest into primary cells, so most primary cell lines require viral infection. A new paper from Niels ...alter protein function or localization. Thus, the usefulness of CPPs must be evaluated on a case-by-case basis...biallelic mutations. Importantly, hESCs retained their stemness, indicating that iTOP may be valuable for CRISPR...

... delete/overexpress endogenous genes or to introduce heterologous genes, but the trend of research has...mimic natural transcriptional programs on multiple genes. Modifying the CRISPRi system for multidirectional...programs to differentially alter the levels of five genes that encode biosynthetic enzymes for violacein, ...

...screens, enabling genome-wide interrogation of the genes required for cellular processes. For a successful...that constitutively express Cas9 and screen for genes that affect Th2 differentiation and activation, ...-LPs and accelerate the discovery of additional genes and small-molecule probes to aid in the understanding...to different lipid compositions of C. elegans membranes, which might beneficially influence the rhodopsin...

...keeping the system to minimal size. To do so, they harnessed the tRNA processing mechanism, which is conserved...lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases, such as muscular...for correcting mutations or installing functional genes to study and potentially treat polygenic diseases... that would perturb the unprecedented number of genes with a single viral vector.   Fig. 1: Schematic...

...medical conditions had been swapped with made up illnesses. They were then asked to evaluate the quality ...methodology makes it easier to spot even minor weakness or limitations in a piece of research (Drummond... mask designs could work to slow the spread of illness. I later helped run workshops where I showed people... jargon that even scientists in neighbouring disciplines might struggle to understand. Why should we trust...

...assignment to discuss goals, expected outcomes, timelines and deadlines. Agree on a way to review project progress...identify constraints and boundaries. Where are the lines of authority, responsibility and accountability?...try "Why Aren't You Delegating" in the Harvard Business Review. Another excellent blog on delegation tips...

...learn new tasks and beat humans at the ancient Chinese game Go). In addition, there was an entire story-telling...neuroscientists are using techniques from across different disciplines to better understand neurobiology and human cognition... provide a highly versatile means of delivering genes to many different cell types with minimal immunological...effects. AAVs are used in experiments targeting genes to specific cell types in the brain, mapping neuronal...

...and funders in the Transparency and Openness Promotion Guidelines; preregistration to increase clarity...variable actually makes a big difference for the robustness of the findings.   How do we make research more...code, and other materials. The use of reporting guidelines can also help remind us to include all of the...

...frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other...PAM sequence that the Cas nuclease recognizes determines site accessibility. Cas9s with a broader PAM ...Joung and Jennifer Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This... sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9...

...cloning uses short sticky ends to insert genes into plasmid backbones, base pairing between these ~4 base ... different types of PCR-generated inserts. By harnessing the power of DNA repair, you can assemble multiple...2024.  References and Resources Further reading Harnessing homologous recombination in vitro to generate...