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Showing: 281 - 300 of 403 results

  1. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... sites, the phage integrates into the bacterial genome flanked by two new recombination sites (attL-left...fragments may be cloned: PCR fragments, cDNA or Genomic DNA and is available for all kind of organisms ...

  2. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ... the genome. A Cas13-based RNA editing system would likely be reversible and would avoid genomic off-targets...

  3. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... FURIN, and more. Recently, scientists used the genome-wide CRISPR libraries, Brunello (knock out) and...Stanley SA, Harris E, Konermann S, Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins...

  4. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...with over 700 views) was an update on new CRISPR genome editing technology, Cpf1. Written by our Science...

  5. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...purity testing, and sterility testing, plus viral genomic sequencing to confirm the identity of the packaged...

  9. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...cells), or they can also be integrated into the genome by cotransfecting them with a plasmid expressing...Lett. 110, 073704 (2017). Hurt, R. C. et al. Genomically mined acoustic reporter genes for real-time in...

  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...alone, and this is expanding as we obtain more genomic data about plasmids (Shintani et al., 2015). Now...22153351 Shintani M, Sanchez ZK, Kimbara K (2015) Genomics of microbial plasmids: classification and identification...

  11. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...  IPTG-inducible expression of T7 RNAP from the genome; does not restrict methylated DNA fhuA2 lacZ:...expresses T7 RNA polymerase (RNAP) from the bacterial genome under control of the lac repressor, which is inducible...

  13. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems. Ahmed A. Gomaa, et...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...sequence, such as the Human papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently quenched (...Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources on Addgene.org Checkout...

  16. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ... Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using...

  18. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...technology, researchers are having trouble getting NgAgo genome editing up and running in their labs. Pooran surveyed... Genes: How to Experiment with Enhancers and Epigenomics 5 Tips for Troubleshooting Viral Transductions...
Showing: 281 - 300 of 403 results