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  1. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...for dynamic experiments in which FRET changes are large. Two other methods are commonly used to measure...

  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...have some disadvantages. These elements are quite large (500-600 bp) and may take up precious space in viral...

  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...using these viruses to mark neuronal connectivity a large majority of neuronal cells are labeled overtime ...based on its unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with AAV vectors...

  8. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...thus dsDNA plasmids are generally preferred for large insertions such as fluorescent proteins or selection...

  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...lead to asymmetrical plasmid loss of the usually large, low copy virulence plasmids. This strategy has ...partitioning machinery away from a larger, low copy plasmid and the larger, low copy plasmid is more likely...

  12. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... 3. Mashiko, Daisuke, et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas ...

  14. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...cells compared to dead cells. As a result, the largely negative live cells can still be easily gated on...

  15. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...Both subunits have short intracellular ends and large ectodomains containing ligand-binding heads and ...

  16. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes and targeting...

  17. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin complexes bound to your...
Showing: 281 - 300 of 390 results