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Showing: 261 - 280 of 283 results
  1. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ... are trying to disrupt or delete, which both inactivates the gene and acts as a marker for the mutation...of experiments it is best practice not to use the same resistance cassette for the mutation and for plasmid...cassettes and your gene of interest is cloned into/inactivates or completely removes (in the case of Gateway...
  2. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... have a higher transduction efficiency, i.e. the same number of AAV particles is able to infect more cells... protects them from antibody recognition and inactivation (György et al., 2014), but scientists have yet...difficult to reach, and are more resistant to inactivation through anti-AAV antibodies. References György...
  3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...approach – co-CRISPR from Craig Mello’s lab – used inactivation of the unc-22 gene as their selection marker...Drosophila S2 cells (Böttcher et al., 2014), inactivation of non-homologous end-joining results in a further...changes 5’ to the PAM, sequences corresponding to the same strand as the PAM produced a higher editing efficiency...elegansGenome with Lethal Mutation Co-Conversion and Inactivation of NHEJ Repair. Genetics 199:363–377 . https...
  4. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...This can differ between fluorescent proteins in the same spectral class (Cranfill et al, 2016). This non-...described by a mono-exponential decay (analogous to radioactive decay). Consequently, the intensity decay is ...comparison of several fluorescent proteins of the same spectral class on the microscopy system and in the...
  5. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...classic approach for studying protein turnover is radioactive pulse-chase experiments. However, these experiments...require radiolabeled amino acids. The use of radioactive materials is highly regulated, requires designated... suspected to interact, are added to a cellular sample. Both antibodies have a short antibody specific...
  6. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...your poster. While a poster generally contains the same sections as a primary research article, it’s important...can be accomplished with an exciting title and attractive figures. Next, the poster should be legible from...four column style, and in all cases the idea is the same; the reader will navigate the poster from column...
  7. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...paying bills and having a life outside my job. The same can be said about teamwork, communication, writing... every piece of work you touch, and be a more attractive candidate for that ideal job. How can you identify...process. You stepped up and got your team on the same page. This came naturally to you. How is this new...
  8. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be... Central PMCID: 5088052. 3. Lentz TB, Gray SJ, Samulski RJ. Viral Vectors for Gene Delivery to the Central...
  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...fluorophore-labeled antibodies with very specific reactivities making them more useful for multicolor imaging...super-resolution STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes...smFPs retain their ability to be detected after sample preparation better than standard proteins tags ...
  10. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...Additional uses The cover letter is a great place to proactively address questions about your application. These...Structure of a cover letter Cover letters follow the same format regardless of whether you’re in academia,...requirements, and address any unusual circumstances or proactively answer questions the hiring manager may have....
  11. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...Green Lab website, but if you don’t see one, be an ACTivist and encourage companies to obtain these labels...cytometers. These fluidic machines handle biological samples and are cleaned between each use. You wouldn’t ...
  12. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...saving freezer space).  The procedure becomes less attractive when noting that one step involves boiling the...less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice, I got good at...
  13. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...the latest fluorescent proteins, I maintain an interactive graph and table of the best fluorescent proteins...be imaged together, you will need to consider the same factors as when choosing an individual fluorescent...
  14. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...modification, or add in fluorescent or chemically reactive side chains, all of which can help determine structure...naturally, rarely are the optimization parameters the same for all three components, meaning that the final...
  15. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...community is loving it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for ...Work with a positive, supportive culture, we are attractive to the local employee pool and we get a lot of...
  16. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...both lentiviruses and gamma-retroviruses use the same gene products for packaging, however, the isoforms...(Plasmid #44246) Expression of a catalytically inactive (dead), human codon-optimized Cas9 under the control...
  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 7, 2024, 2:15 p.m.
    ...that available and accessible weren’t always the same thing shaped both Addgene’s founding and growth ...design in our digital content. We have implemented proactive measures to find potential barriers that may not...
  18. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...their disciplines. Included in the article is an interactive chart where you can see the yearly interdisciplinarity...amount of interdisciplinary research while at the same time increasing interdisciplinary citations in their...
  19. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based approach... mechanisms and consequences of cohesin gene inactivation in human cancer.     References 1. Russell, ...
Showing: 261 - 280 of 283 results