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Showing: 261 - 280 of 499 results

  1. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ..., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene... Percent of Neurons Transduced   Dorsal Root Ganglion (DRG) AAV.9 (parental) 46 +/- 0.7% AAV.PHP.S...

  2. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...

  3. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...figure. Some follow the same figure by figure organization with a different person responsible for each...journal clubs should be well planned and carefully organized. Most importantly, they should be regularly assessed...

  5. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...these vectors for gene therapy. In 2003, VIRxSYS began the first clinical trial with a lentiviral vector... vector. Naldini L, Blömer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. Science. 1996 Apr...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...their suppliers, and public health and medical organizations are working to help educate the public about...chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and...

  7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...directly from A. Victoria and expressed in any organism while still maintaining fluorescence. The protein...soon as its sequence was elucidated, scientists began engineering new versions of GFP through mutagenesis...

  8. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...

  9. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...plant tissue cultures.   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene ...transgenic plants is a common problem in non-model organisms. Therefore, transient methods are usually the ...

  10. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered...hottest antibodies in Addgene’s collection since we began distribution and has been verified to perform well...

  11. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...

  12. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor...mTurquoise2, not shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is...

  13. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...

  14. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...

  15. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022). The oxytocin sensor MTRIAOT ...various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP to urotensin. The MTRIA...

  16. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. CRISPR...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...

  19. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies...
Showing: 261 - 280 of 499 results