...remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C. elegans were released, signaling...revolution!   References Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette....strategies for CRISPR/Cas9 editing in C. elegans: Engineering the Caenorhabditis elegans genome using Cas9-...

...since the very beginnings of the field. Those engineered sensors were designed to replace synthetic calcium...problem that he considers “one of the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors...trial and error. Transition metals also present challenges in that they are found in cells at much lower...

...sources of information if those sources affirm or challenge their beliefs. I asked participants to read short...read an abstract for a study with findings that challenged their beliefs, they were much more likely to ...dissonance (Kunda, 1990). More specifically, they were engaging in a behaviour called the “biased evaluation of...

...initially limit the technology to research centers engaged in long-term, mandatory follow-ups and observational...Download the CRISPR 101 eBook Read our genome engineering blog posts Resources on Addgene.org Read our...CRISPR plasmids by function Check out our genome engineering guide ...

...recombination-mediated genetic engineering (find more information on bacterial engineering using CRISPR on our blog...interactions. Combining synthetic biology with engineering: glowing bacteria and a fish robot to detect ...

...  7 ways to strengthen your new relationships Here are some ways to go about strengthening a new relationship...often the easiest way to build relationships and strengthen them over time.  Things to avoid when following...

...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...environment.  Be kind to yourself. This is a challenging period for everyone for numerous reasons, and...check out She Geeks Out, Science, or Resources to Engage Kids at Home. It’s normal and okay to feel overwhelmed...

...newborn gut. One of Fatima’s ultimate goals is to engineer bacteria for the efficient product of HMOs that...surfaces. After incubating the sensor with bacteria engineered to produce HMOs, sensor binding results in an...future work with HMOs will enable researchers to engineer healthier microbiomes in people all over the world...

...seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...enhanced normalization of read count and fragment length across samples, NGS data obtained via plexWell™...

...Cofactors/Substrates Required Not Required Signal Strength Lower Higher Sensitivity Higher Lower Background..., substrate requirements and photon emission wavelengths. Renilla luciferase from the sea pansy (Renilla...these luciferases have been subjected to further engineering to improve upon the properties of the native ...

...many career pathways exist for scientists and engineers, a few brave scientists will choose to start their... interdisciplinary, with emphasis on genetic engineering, sequencing, and translation of results to companies....” Take home message Starting a lab can be a challenging process. Each PI emphasizes the importance of...

...pGGA008 (AlcA) and pGGC011 (AlcR) from red flame GreenGate kit Steroid-regulated LexA promoter XVE (synthetic...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light... induce transcription of your target gene? The strength of inducible promoters can vary a lot. If you’...

...reacting to a chemical, but by emitting a specific wavelength of light in response to excitation by a laser... degrading them. You’ll also need to know the wavelength of laser needed to excite them - and it’s a good...   Fig 2: Try to inversely pair fluorophore strength with protein abundance.    Second, you’ll ...

...fluorescent as well as FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging...biosensors? Well, a lot of these sensors are already engineered into viral vectors, so if you want to use them...Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience...

...and tractable tools for genome engineering. Scientists have engineered viruses to target specific cell...infect. In the lab, this means viruses can be engineered to be cell type specific. Once inside the host...

...Non-Continuous Evolution (PANCE) selection systems to engineer better and faster ABEs (Fig. 2). These systems...ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted evolution ...Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR (2020) Phage-assisted...

...Immunologists are coming together to tackle the challenge of curing cancer in cross-disciplinary projects...CRISPR/Cas9 methodology with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor..., CRISPR/Cas9 genome editing allows targeted engineering of the TCR locus. He also highlighted the UCSF...

... on differences in emission wavelength. Filtering the emission wavelength partially separates the signals...In fluorescent imaging, you use the excitation wavelength to specify which FP emission you’ll capture. ...

...this virus. While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9...LentiCas9-blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al...makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability...

...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...