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We narrowed to 676 results for: plasmid

Showing: 241 - 260 of 676 results

  1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...Biotechnology which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen....with other fluorescent proteins. Find mNeonGreen plasmids here! mTFP1: a monomeric teal fluorescent protein...physiologically relevant changes in pH. Find mTFP1 plasmids here! mWasabi: a bright green fluorescent protein...excite at 400 nm while EGFP does. Find mWasabi plasmids here! One of the most notable things about these...are best suited for certain studies. If you have plasmids containing mNeonGreen, mTFP1, or mWasabi that ...with the scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "...collection Check out Addgene’s fluorescent protein plasmids and resources Find fluorescent protein kits ...

  2. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...is mediated by ADAR2DD, not PspCas13b. Find the plasmids for the RESCUE RNA editors here! Testing and improving...for viral delivery.  Find the RESCUE RNA editing plasmids here! Future directions Building on previous RNA...Science (2017):pii: eaaq0180. PMID: 29070703. Find plasmids from this paper at Addgene. Abudayyeh, Omar O...550(7675) (2017):280-284. PMID: 28976959. Find plasmids from this paper at Addgene. Abudayyeh, Omar O...27256883. PubMed Central PMCID: PMC5127784. Find plasmids from this paper at Addgene. East-Seletsky, Alexandra...) (2016):270-273. PubMed PMID: 27669025. Find plasmids from this paper at Addgene. Gootenberg, Jonathan...28408723. PubMed Central PMCID: PMC5526198. Find plasmids from this paper at Addgene. East-Seletsky, Alexandra...

  3. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ... Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids at Addgene Check out additional...other species.  When he found the chromoprotein plasmids available from Addgene, he knew it would be a ...molecular biology. “What I'd like to do with the plasmids from Addgene is have the students transform them...plate skills? Find the chromoprotein expressing plasmids here, and check out our streak plate protocol ...

  4. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

    Type
    Blog Post
    Published
    Dec. 8, 2015, 3:30 p.m.
    ...23953120. PubMed Central PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan...2015): 1334-1341. PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on...Blog Read our Recent Blog Post on GFP Activable Plasmid Tools Being Developed in the Cepko Lab Listen to...Addgene Website Find Cepko Lab Plasmids at Addgene ... plasmid tools...

  5. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...1). Plasmids: We generated a series of plasmids that are available from Addgene. These plasmids enable...Resources on Addgene.org Browse all CRISPR plasmids CRISPR plasmids for protein tagging Find validated gRNAs...Detailed protocols describing the use of these plasmids are available here. The Methods section of Nabet...cloning procedures used to construct the deposited plasmids. Molecules: We synthesized dTAG molecules by conjugating...terminus should be tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality...Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in human cells Resources...

  6. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...Addgene.org Find plasmids from the Bethune Lab Find Plasmids from the Roux Lab Find Plasmids for FRET ...BirA* fusion proteins are expressed in cells via plasmids or by the creation of stable cell lines and these...Interested in using Split-BioID with your research? Plasmids are available from Addgene! References Schopp... Central PMCID: 5467174. Find the Split-BioID Plasmids Here! Munter, S.D., Görnemann, J., Derua, R.,...site than in Schopp et al. Find these Split-BioID Plasmids Here!   Burke, B., Kim, D.I., Roux, K.J., & Raida...PubMed Central PMCID: 3308701. Find the BioID Plasmids Here! Macneill, S., & Varnaitė, R. (2016). Meet...techniques for visualizing protein turnover Learn about plasmids for in vivo biotinylation of bacterial fusions...

  7. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...insight to the most requested plasmid, the number of requested plasmids, or trends of requests over time...website, allowing depositors to download their plasmid request data, as a CSV file, anytime. The CSV is... Visualizing the data So, after downloading my plasmid request data, I started to explore how I could ...file is open for anyone to use.  View the Addgene Plasmid Request Data Dashboard! Figure 1: The ...

  8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair template...of Addgene plasmid options for templates - see the example at right using blue flame plasmid pmScarlet_...desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N ...out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression ...pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology arms and...template! If you have a plasmid with your desired insertion feature (e.g., a fluorescent protein), you can...described in Figure 2.  You can also use Addgene plasmids to purify Cas9 protein. The table below contains...

  9. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...Turquoise (CeNL; Plasmid #85199), Yellow (YeNL; Plasmid #85201), Orange (OeNL; plasmid #85202) and Red ... Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the...specific strategies and tactics for getting the plasmids he wanted. Addgene makes it so much easier to ...kDa. An example is the CRISPaint-NLuc construct (Plasmid #67178) for use in the system outlined in Schmid-Burgk...CyOFP1-NLuc-CyOFP1; Antares). Direct injection of plasmids for Antares or firefly luciferase into mouse livers...Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions of.... This construct is available through Addgene (Plasmid #83926). Live-Cell Voltage Sensor  Ingaki and ...

  10. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ... in E. coli with two plasmids (Figure 3 below): Input Plasmid: The input plasmid (high copy, ColE1 origin...inducible promoter (PPhlF).  Output Plasmid: The output plasmid (single copy, bacterial artificial chromosome...through Addgene. Their plasmid maps and GenBank files can be found on their plasmid pages. All two-input...indicated figures in our paper (1)) Input Plasmid Output Plasmid Fig. 3A pNR64 pNR160 Fig. 4A pNR220...two-input RSMs and three three-input RSMs. The plasmids used to construct these RSMs are listed in the...paper (1). These parts are also annotated on the plasmid maps and GenBank files uploaded to Addgene. We ... Visit Our SynBio Pages Check Out More Lu Lab Plasmids Find More Genome Engineering Tools  ...

  11. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Human whole genome...attached fluorescent protein to the DNA. They created plasmids for purification of tTALE-eGFP and tTALE-mCherry...digestion of single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., Sci. Rep. 2019...

  12. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...at the cells on the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, ...colonies to determine which cells have taken up the plasmid. Of course, other follow up methods like sequencing... chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed, asPink, spisPink, scOrange,...chromoproteins. In some cases, cells containing high-copy plasmid-borne chromoproteins lost color overnight in liquid...problem. There is no single “ideal” chromoprotein plasmid that combines the most desirable features: intense...Chromoproteins for allelic exchange Figure 3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus...sequential homologous recombination events: The plasmid carrying the desired genetic modification integrates...

  13. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...summer or just a bright new plasmid. One area with lots of bright new plasmids is our Fluorescent Biosensors...SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3 plasmids here! Find viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.... 2022, under CC-BY-NC license.   Find JEDI-2P plasmids here! Find viral preps here: pAAV-EF1a-DIO-JEDI...et al. 2022, under CC-BY license.   Find pYtags plasmids here!   Farahani PE, et al. pYtags enable spatiotemporal... suitable for AAV packaging.    Find iGECInano plasmids here!   Matlashov ME, et al. Design and Initial...CC-BY-NC-ND license.   Find KRaION and Kbp sensor plasmids here!   Torres Cabán CC, et al. Tuning the Sensitivity...et al. 2022, under CC-BY license.   Find LiLac plasmids here!   Koveal D, et al. A high-throughput multiparameter...

  14. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...and to model diseases in vitro. Find stem cell plasmids at Addgene Many sources of adult somatic cells...Episomal vectors- extrachromosomally maintained DNA plasmids that express reprogramming factors To date, the...group is an optimized mixture of three episomal plasmids (Table 1) encoding the reprogramming factors. ...enhance the reprogramming efficiency, an extra plasmid, pCXWB-EBNA-1 is used in the reprogramming cocktail...increases protein expression from the other episomal plasmids. When used on PBMCs, the pCXLE episomal vector...available at Addgene. Table 1: List of pCXLE episomal plasmids, their Addgene ID numbers and the reprogramming...reprogramming factors they encode Episomal Plasmid Addgene ID# Encodes pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL...

  15. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ....org Find Cre-Lox Plasmids Find Optogenetics Plasmids Find Chemogenetics Plasmids ...vectors expressing light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience...neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP) and the...recombinase. After in-utero electroporation, this plasmid mix results in sparse labeling of cortical neurons...labeling of individual neurons one could reduce the plasmids amounts to reduce transfection efficiency. However...visualized at the same time from two different plasmids this approach becomes problematic as it reduces...that most cells are transfected with all labeling plasmids and the low concentration of recombinase restricts...

  16. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...will be able to request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five... more.  What will be available?  Over 3000 AAV plasmids in the Addgene repository will be available for...Packaged on Request service. A small subset of AAV plasmids will have technical or legal restrictions that...both our depositors and requestors.  Requesting Plasmids available for Packaged on Request consideration...recommend you check the original paper of your AAV plasmid of choice for more information on its performance...performance.   Titers We expect titers for plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013...

  17. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...Addgene distributes several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags, protease...without the artifacts sometimes associated with plasmid-based overexpression. Tim Formosa, at the University...). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site in place of the epitope...Jürg Bähler have deposited a large collection of plasmids for genome modification in yeast developed by ...Bähler et al.(2) describe a modular collection of plasmids for a wide variety of genome modifications in ...Longtine et al.(3) describe a complimentary set of plasmids for use in S. cerevisiae, with the additional ...

  18. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...from our repository to find our most requested plasmid in 2015. In line with all of the exciting developments... past year, we're excited to announce that the plasmid most requested from the Addgene repository in 2015...put to such great use. It’s just one of the many plasmids being distributed to and accelerating research...Knockouts Read Some Common Ways to Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website...Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries ...

  19. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids 101: Green Fluorescent Protein Resources...Fluorescent Protein Collection Find Plasmid Kits at Addgene Find Plasmid Cloning Protocols References 1....combination of different genetic sequences into plasmid DNA. In high throughput cloning techniques, although...MXS chaining MXS-chaining was designed to create plasmids for fluorescence imaging or flow cytometry applications...modules (Figure 2) are contained in individual plasmids and are each flanked by the same multiple cloning...throughput cloning is to facilitate the construction of plasmids with many components. The creators of the MXS-... plasmid cloning...

  20. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...relevant plasmid depositors and their institutions to distribute viral preps derived from plasmids in the...largest new initiative since we started distributing plasmids in 2004. We’ve already distributed over 500 viral... virus. Addgene is a 501(c)3 nonprofit and our plasmid service was started based on donations. That seed...depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently sharing viral preps) through...positive response to the option of Addgene providing plasmids in a ready-to-use viral format. Having identified.... With their expertise in shipping hundreds of plasmids a day, the Lab and Office Teams develop shipping...
Showing: 241 - 260 of 676 results