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  1. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...organisms, they can also be used to easily screen multiple therapeutic compounds in one experiment. Zebrafish...making it easy to conduct large experiments over multiple generations. Similarly to other model organisms...

  2. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...of the promoter as part of a multiple cloning site (MCS). Having multiple sites allows you to easily orient...

  3. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...and reprobed with different antibodies allowing multiple targets to be assessed from a single western.  ...used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the primary ...

  4. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... any gene of interest, one should require that multiple gRNAs of different sequences give rise to the ... in the interpretation of results by requiring multiple sequences targeting a gene to score in order to...

  5. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...goal of isolating single cell clones to use for multiple downstream experiments. You only successfully ...most commonly used Cas, spCas9, has gone through multiple rounds of optimization which have led to more ...

  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ... genome amplification (by a mechanism known as multiple displacement amplification) even in the presence... of 10-20 colonies using primers bordering the multiple cloning site of the vector. In order to reduce...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...a Group Leader position, what will you need? Multiple first or corresponding author publications (published...interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the end of grad...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...you can see, AAVs of each serotype can transduce multiple tissues. So how do you choose the right serotype...thawing. Although more stable than other viruses, multiple freeze thaw cycles can still reduce AAV titer....

  10. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...antibody works! Multiple (Independent) Antibodies Strategies These strategies use multiple, unique antibodies...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...be beneficial when expression levels are low as multiple secondary antibodies can bind to a primary antibody...causing dead cells to fluoresce.  When running a multicolor flow cytometry experiment, users must also prepare...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...J., Deverman, B. E., & Gradinaru, V. (2020). Multiplexed Cre-dependent selection yields systemic AAVs ...

  14. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...new sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated...

  15. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of ...

  17. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...the twelfth day of CRISPR, Addgene gave to me: multiplexed gRNAs.  We hope you’ve been enjoying our CRISPR...

  19. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ... Addgene. The kitchen and office areas provide multiple streams for composting and recycling in the event...

  20. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...library of gRNAs with a CRISPR nuclease target multiple genes in a cell population. The resulting mutant...
Showing: 241 - 260 of 455 results