...researchers involved. Communication is a major challenge when researchers are communicating across disciplines...disciplines or doing different tasks. But these challenges can be exaggerated when communicating across ... help other teams successfully overcome these challenges, too. Schedule regular meetings Scheduling a...

...them attractive tools for the life scientist or bioengineer.   Figure 1: Map of a plasmid with its...reporter plasmids, viral plasmids, and genome engineering plasmids. Some of the many things that plasmids...controlled changes to an organism’s genome (genome engineering) Produce synthetic viruses that can be used in...

...fluorescent protein-based sensor. This sensor was engineered from a GFP variant and a DA receptor such that...PMCID: PMC3083031. Kebabian, John W., and Paul Greengard. "Dopamine-sensitive adenyl cyclase: possible ... Kebabian, John W., Gary L. Petzold, and Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate...

...cycle of one step. Challenges in the LEGO science video process One major challenge when we started was...will bring people together and make these daily challenges a little easier to overcome. We want to encourage...

...Kurt Swanson a structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in...FOP until a cure is available. Addgene and AJ's Avengers This year Addgene, where my wife Lianna has made... Swanson is a structural biologist and protein engineer currently working at Sanofi Pharmaceuticals in...

...the Rluc8 was more challenging. The Roth lab used structure-guided protein engineering and experimental ...the blog post. References Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin...

.... The resultant JEDI-2P sensors overcome many challenges in 2-photon in-vivo imaging of voltage fluctuations...neurons, far out-performing previous attempts to engineer potassium-selective channelrhodopsins. HcKCR1 ...work also proposes promising avenues for future engineering to shift the spectra and improve the performance...

...remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C. elegans were released, signaling...revolution!   References Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette....strategies for CRISPR/Cas9 editing in C. elegans: Engineering the Caenorhabditis elegans genome using Cas9-...

... fiber (Park et al., 2019). This provided an engineering road map of sorts, giving an idea of the stiffness...whenever necessary. This is all an impressive engineering feat, but you’re probably wondering “did it actually... Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC), 4322–4325...

...could potentially be used to report upon and/or engineer “temporally ordered biochemical events” such as...RNA-based toehold switches described in this post are engineered to bind RNA sequences derived from Zika virus..., Multitasking “CRISPR-OS” In this post, Albert Cheng and Mark Wanner from the Jackson Laboratory for ...

...Enhancement in vector stability." Biotechnology and bioengineering 94.2 (2006): 322-329. PubMed PMID: 16528756...pressure and sodium butyrate." Biotechnology and bioengineering 42.5 (1993): 601-610. PubMed PMID: 18613082...and antibody production." Biotechnology and bioengineering 37.10 (1991): 989-993. PubMed PMID: 18597326...

...since the very beginnings of the field. Those engineered sensors were designed to replace synthetic calcium...problem that he considers “one of the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors...trial and error. Transition metals also present challenges in that they are found in cells at much lower...

...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...environment.  Be kind to yourself. This is a challenging period for everyone for numerous reasons, and...check out She Geeks Out, Science, or Resources to Engage Kids at Home. It’s normal and okay to feel overwhelmed...

...seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...enhanced normalization of read count and fragment length across samples, NGS data obtained via plexWell™...

...  7 ways to strengthen your new relationships Here are some ways to go about strengthening a new relationship...often the easiest way to build relationships and strengthen them over time.  Things to avoid when following...

...initially limit the technology to research centers engaged in long-term, mandatory follow-ups and observational...Download the CRISPR 101 eBook Read our genome engineering blog posts Resources on Addgene.org Read our...CRISPR plasmids by function Check out our genome engineering guide ...

...recombination-mediated genetic engineering (find more information on bacterial engineering using CRISPR on our blog...interactions. Combining synthetic biology with engineering: glowing bacteria and a fish robot to detect ...

...sources of information if those sources affirm or challenge their beliefs. I asked participants to read short...read an abstract for a study with findings that challenged their beliefs, they were much more likely to ...dissonance (Kunda, 1990). More specifically, they were engaging in a behaviour called the “biased evaluation of...

...newborn gut. One of Fatima’s ultimate goals is to engineer bacteria for the efficient product of HMOs that...surfaces. After incubating the sensor with bacteria engineered to produce HMOs, sensor binding results in an...future work with HMOs will enable researchers to engineer healthier microbiomes in people all over the world...

...Cofactors/Substrates Required Not Required Signal Strength Lower Higher Sensitivity Higher Lower Background..., substrate requirements and photon emission wavelengths. Renilla luciferase from the sea pansy (Renilla...these luciferases have been subjected to further engineering to improve upon the properties of the native ...