Google Forums Round Up: First Impressions of NgAgo
Type
Blog Post
Published
Aug. 4, 2016, 2:30 p.m.
... PMID: 27136078.
2. Burgess, Shawn., et al. "Questions about NgAgo." Proteins & Cell (2016).
3. "NgAgo...engineering methods have their own pros and cons (2). The NgAgo DNA guide is inexpensive and can be 5’...degraded by the cellular machinery, leaving a small amount of active NgAgo/ssDNA complex in the cell. As proposed...results of Gao et al 2016 in a letter to Protein & Cell.
Update (August 3rd, 2017) THE ORIGINAL NgAgo ARTICLE... guide, it cannot be produced from a plasmid in cells. Additionally, in vitro assembly of NgAgo/ssDNA ...dangerous, non-physiological temperature for mammalian cells and one which diminishes the endonuclease activity...guide. Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides and an NgAgo expression plasmid...