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Showing: 241 - 260 of 506 results

  1. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...protocol, the size of the cuvettes your lab’s electroporator uses, etc. The DNA amount can be anywhere from...Screening Zhang Lab - Multiplexed Overexpression of Regulatory Factors (MORF) Library 3,548 250 - 10,000...

  2. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...additional protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based...of auxin to the culture medium. Any number of regulatory sequences, including protein enhancers and repressors...

  3. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...in real time! I follow great online science communicators like Phil Plait @BadAstronomer, Emily Graslie...when I was trying to find local informal science educators, and ever since, the State Museum has been the...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...viral vectors) without triggering a destructive inflammatory immune response. At the time, adenoviral vectors...choroideremia, X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing...

  6. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...four examples of technologies pioneered in his laboratory. Learn more here. Additional resources on the...

  8. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities...

  10. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ... region is transferred into the plant. In the laboratory we can utilize this natural process by replacing...and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration can often be seen by...

  11. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar and Marty, Jean...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...

  13. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...  “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...

  14. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc), simian virus 5-derived...usually provided by red fluorescent proteins such as tdTomato or RFP or blue fluorescent proteins like Cyan....

  15. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...example, neurons treated with a phorbol ester activator gave fluorescent signal in the ticker tape from...transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD...

  16. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS...

  17. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first...Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389...

  18. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal...

  19. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...own replication machinery such as Rep or other initiator proteins, they do not require host proteins for...

  20. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...signal inside the tumors was overlaid with the anatomical B-mode images in greyscale.   ARG expression...-specific ultrasound signal is in hot and the anatomical B-mode images are in greyscale. (scalebar = 2mm...
Showing: 241 - 260 of 506 results