Antibody Validation for Flow Cytometry
Type
Blog Post
Published
Sept. 10, 2024, 1:15 p.m.
...protein level at the timepoint studied
Off-target activities of the RNAi
Correctly interpreting your data...the abundance of the epitope in the sample, relative to cross-reactive antigens – that is, an antibody shown... recognize different epitopes of the same protein, show the same pattern of labeling, this is supportive...detecting the target-of-interest in your assay and sample-of-interest. The approaches available broadly map...determining the selectivity of antibodies for other sample types and for non-surface (intracellular) antigens...approaches used will be different depending on the sample and target type.
To start the validation process...the target at a robust level, similar to that in samples of interest. If you are working with cells that...