...groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking...address this issue, but it still points to a far larger and more complex problem endemic to biomedical ...

...Permeabilization What is the point?  Antibodies are large proteins that need a little help crossing cell membranes...

...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience...

...amplicons.  Sybr has high background noise, due to the large amount of dsDNA in most starting materials, and ...

... homology directed repair (HDR), and generating large genomic deletions. What are the advantages of Cas9...

...believe this isn’t cause for concern. However, a large number of hits to one sequence/gene - we set our...

... advantage of this technique is the potentially large number of single clones obtained (on occasion we...

...Tips and tricks from the bench Don't pick too large of a colony. Too many bacteria can inhibit your ...the insert site. A positive clone will produce a larger size product then a negative clone without the ...-) will not. Backbone-specific primers (2) give larger sized products for positive clones (+) compared...

... parts. That was the motivation for releasing a large parts kit. Once you have so many parts available...

... called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George...discovery, the family of FPs just keeps getting larger with hundreds of variants available. Read on to...

...commonly used to create functional knockouts or large deletions, the ability to analyze and interpret ...

... are just 2 examples of organizations that hold large, annual conferences. Gordon conferences offer more... Career Expo and the ACS career fair, which are larger meetings offering resources for students seeking...

...experience. With this valuable training behind you (and largely only that is necessary), there are opportunities...to manipulate cells (manufactured by one of the larger biotech companies), and as far as I remember, it...

... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker...

...pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL). The standard...

...editor experimentalist, and especially shines once large-scale prime editing experiments come into play. ...

... did, and state that it would help you manage a large number of files as part of the job. Structure of...

... Design and selection for targets can be done largely in vitro; Easy and reproducible commercial synthesis...metal ion, or fold to incorporate themselves into a larger target, such as a protein. Such flexibility makes...

..., to search the literature, and to synthesize a large volume of information: basically, a scientist’s ...

...Gibson assembly method Single reaction, can assemble large DNA sequences, no restriction enzymes needed Not...