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Showing: 241 - 260 of 499 results

  1. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...mammalian cell lines, Drosophila cells, and C. elegans. In addition to generating fluorescent protein .../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources...

  2. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses...experience from the perspective of both grassroots organisations that represent postdocs and institutes that ...

  3. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...work or volunteer for a science communication organisation like Building with Biology. Local environmental...You can draw live sketches at conferences like @ATJCagan or create agar art with colorful microbes? Try...

  4. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...Green. Zika background First identified in 1947 in Uganda, the Zika virus had received little attention and...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...

  6. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...you can accomplish this by employing a neat and organized format.  Columns. You can choose from either ...Mike Morrison, a psychology doctoral student at Michigan State University. The idea is that the main research...

  7. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...

  8. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor alpha (Esr1...

  9. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...

  10. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015...variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team ...

  11. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...sea anemone (Chan et al., 2006). Why do these organisms have chromoproteins? One reason may be that chromoproteins...symbiotic algae, during heat stress. For other organisms, like the sea anemone, scientists think that chromoproteins...

  12. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc., which will...target protein and an E3 ligase through interacting ligands. After an E3 ligase facilitates ubiquitination ...

  13. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...development analyst, I negotiate with global organizations that partner with Addgene. What are the most...

  17. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...

  19. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...

  20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ..., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene... Percent of Neurons Transduced   Dorsal Root Ganglion (DRG) AAV.9 (parental) 46 +/- 0.7% AAV.PHP.S...
Showing: 241 - 260 of 499 results