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Showing: 221 - 240 of 455 results

  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...attention to antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they...Antibiotic Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite...

  2. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...  Skills such as research, collaboration across multiple disciplines, writing, project management, and ...knowledge of the 8 core competencies through a multiple choice exam.  The bottom line on being a grants...

  3. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...depicted in the accompanying cartoon, they have a multitude of uses including (but not limited to) purification...localizes your protein to the nucleus? Or you want multiple rounds of purification to get your protein as ...

  4. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...interdisciplinarity index of research for single or multiple disciplines – you can select the disciplines and...networking, visual appeal, instant sharing across multiple other sites), I created a new platform for sharing...

  6. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...researcher exquisite control over expression of multiple genes in specific cells at specific times. Many.... “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit ...

  8. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...inherent in Nextflow’s design, we were free to use multiple programming languages (Python, Perl, Java, R, ... adaptable property gave us the freedom to use multiple open-source sequence assembly tools at the appropriate...

  9. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... the more complex Class I systems that contain multiple subunits, but the more they worked with it, the...high-fidelity rate. Both Cas7-11 and PASTE open up multitudes of possibilities at the bench and potentially...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...

  11. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII ... Proteins which contain hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble...

  12. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...behavioral geneticists may track the motion of multiple animals on different occasions via video, generating...limiting numbers of figures such that many have multiple, complex panels. PLOS, itself, has not finalized...

  13. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...be packaged in AAV vectors, potentially with multiplexed targets.   Figure 4: CHARM uses a fusion...  Multiple Authors...

  15. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ... scrambling. Everything we had set up involved multiple people in tight locations. Conference rooms for...to the talent’s shirt. But knowing I would have multiple interview subjects, I positioned a boom mic just...

  16. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...These methods can include labeling markers with multiple proteins/antibodies, targeting markers found within...Otherwise, your secondary antibody will bind to multiple primary antibodies, yielding a false positive ...

  17. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination Induced by Double-Strand... Multiple Authors...

  18. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... words, any insertion or deletion that isn’t a multiple of three will do. A popular screening method for... Multiple Authors...

  19. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...every communication that gets sent out through multiple channels is not reasonable or sustainable. That...will be gray areas that either a) overlap with multiple teams or b) fall into a gap between teams that...

  20. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...obtaining homo- and heterozygous mutations by multiplying the single allelic mutation incorporation probabilities... systems.     References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." ...
Showing: 221 - 240 of 455 results