...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...for a successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral experience...data needed to rule out alternative models or to strengthen your new model?  PFs need to be regularly thinking...and answering questions. Organizing a thoughtful engaging talk takes significant effort. It requires evaluation...

...the nervous system, and vectors for zebrafish engineering. You can find additional hot plasmids in our ...Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

... cytokines like TGFβ and IL-6. However, when strengthened through efforts to boost the activity of the... form of immunotherapy in recent times. These engineered T cells are capable of recognizing targets like...the above therapeutics, techniques like genome engineering have played a huge role. For instance, researchers...at Binghamton University. She works on genome engineering and cancer immunotherapy, and is interested in...

...-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production of functional...are between 5-8 amino acids long, with a typical length of 5 or 6 amino acids (Cruse et al., 2004). Because...natural immune system production and many more engineered methods. Each type of antibody and production...

...the recent Keystone Meeting "Precision Genome Engineering and Synthetic Biology". His reflections on the...example, the authors Patrick Hsu, Eric Lander, and Feng Zhang acknowledge the role of data and materials...online user forums such as http://www.genome-engineering.org and http://www.egenome.org." At the Keystone...

...joachimgoedhart.   Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... genome engineering...

...becoming an ever more ubiquitous tool for genome engineering, and studying its structure can help us understand... it’s the reason guide sequences have a maximum length of 20 bases: nothing longer will fit. Stabilization...structural insight also spurs the next generation of engineered CRISPR enzymes with their own tradeoffs. And ...the Addgene blog Components of CRISPR  xCas9: Engineering a CRISPR Variant with PAM Flexibility Cas9 vs...

...🎄🎁🎅🤶🎄 pic.twitter.com/rDaEjxTNZX— Heng Chooi (@YitHengChooi) December 4, 2021   I spent a year hoarding...used tip boxes for a lab supply tree and set a challenge to my lab mates. They did not disappoint. #deckthelab...

...completing her PhD in Chemistry and Chemical Engineering with Harry Gray at Caltech, Crystal moved on ... @ShihCrystal.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...

...to face global challenges, we hope to empower those that need to face these challenges locally. We look...themselves on the front lines of emerging global challenges. However, these same researchers face significant...

...synthetic biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's...projects in Ghana.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

... the Collins' lab at the time) got the idea to engineer bacteriophage (viruses that infect bacteria) for...grow with his help! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

....     Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform called...efficiency and specificity of multiple wild-type and engineered variants of Cas nucleases. In doing so, they ...created a modular toolkit for Cas-mediated genome engineering in plants. Read the paper in bioRxiv Find...

... up with it. The peer review process has been challenged with many closely spaced CRISPR software submissions...facilitate most experimental applications in CRISPR engineering. When compared to that collective utility, it...This is only one of many decisions faced by those engaging in CRISPR design, but it is a significant hurdle...limitation, but the tool is fast enough that, if you’re engaged in heavy CRISPR design (I’m talking screens and...

...exciting developments surrounding CRISPR genome engineering in the past year, we're excited to announce that...lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute and was designed...PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries...

...My son is an engineer and my daughter has a business degree. From the very start of their undergraduate...talent in science related careers. Failure to strengthen the talent pipeline sets the world up for reduced...Image from pd|hub.   To begin to tackle these challenges, a group of stakeholders came together with the...

...genome edits compared to rat APOBEC1. Now, they’ve engineered SECURE adenine base editor variants using a protein-truncation...activation-induced cytidine deaminase (AID) and an engineered tRNA adenosine deaminase (TadA) to a catalytically...packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang’s lab previously developed a programmable ...

... some challenges during my internship, but nothing could have prepared me that those challenges would ...exploring my different interests but also a way of challenging what I am used to. Brandon Solis, marketing...

...be removed by re-expressing the transposase. A challenge of using PB to generate iPSCs is that genomic ...the cytoplasm independent of the cell cycle. A challenge of using Sendai is that since it’s replication...clones tested had plasmid integration. Another challenge is that multiple transfection makes it difficult...induced pluripotency: Recent progress and future challenges." Journal of Cellular Physiology 228, no. 2 (...

...co-op) is what has brought me, a second year bioengineering student, to Addgene. I am currently working...interview. I anxiously prepared for the call. The length of this interview was intimidating to me, as I ...comfortable.     Lucie Wilson is a rising junior and bioengineering student at Northwestern University. She is ...