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  2. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...you see that there is high RNA expression in the retina and in parts of the brain, but almost none in the..., you will see a strong antibody signal in the retinal sample, but little to no signal in the liver sample...

  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no....

  5. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... proper behavioral readout that allows you to interpret the results of your experiment. Like many things...optogenetics has already been proven. Therefore, a concrete, well defined, and robust behavioral output is...

  6. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...editors have an activity window restricted to the stretch of ssDNA created by Cas9 binding. Pro tip! In CRISPR...editors (calling them GBEs), Xueli Zhang’s lab, returned with a unique approach to improving C → G editing...

  7. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    .... Other techniques, like electron microscopy and FRET, can offer more information on these regions.   ...SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo et al., 2022). ...

  9. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...when using fluorescent probe-based chemistry - theoretically, up to five or six reactions in a single well.... Epub 2009 Feb 26. PMID: 19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. ...

  10. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...has both high binding capacity and high protein retention. It comes in a variety of pore sizes and protein...with high binding capacity, though it has lower retention after binding and washes. It’s less sturdy and...

  11. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ... rule of thumb, the more strictly you want to interpret your blot’s data, the more time you’ll want to...starting a western blot, think about how you’ll interpret the data and what level of accuracy you’ll need...

  12. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...promoter sequence. Should one of these random stretches of DNA contain an enhancer, it will activate its...GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used...

  13. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this...

  15. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ... This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence, autophagy...

  16. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...protein purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists A new...

  17. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...box in the copy area. These spent cartridges are returned to Xerox for recycle and reuse, reducing the need...

  18. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV Retrograde (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL...

  19. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ... the target region, if the report describes a retrograde serotype An abstract that summarizes your experiment...
Showing: 221 - 240 of 471 results