...friendly, to own the process entirely remains a large undertaking. Priority number one was to ensure that...regions that are GC rich. Since NGS assembles a larger sequence from many smaller sequences, regions with...

...cells with unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed...without any reduction in performance compared to larger genome-wide libraries. Find the library here ...

...sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore or...

...at two sites within the viral genome to excise a large portion of it. This ongoing trial is sponsored by...

...could be good choices if you have a particularly large number of inserts to assemble. You could also consider...

...the possibilities can seem overwhelming given the large assortment of fluorescent proteins available. Fortunately...

...CRISPR variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming...

...in my lab notebook from time to time. I wrote in large letters “Going for it!” We kept our day jobs and...

...Conversely, if your protein of interest is large, look for a larger pore size (0.45 µm).  Types of transfers...to overnight to run, and is recommended for very large proteins. A semi-dry transfer is faster — 3–30 minutes...

...can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells...

...system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created...

... prone to recombination, while a library with a large backbone might be more difficult to transform.  ...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in...

...proteasome for degradation. The HaloTag is quite large (33 kDa) and does not self-cleave off of the protein...

... This specific example is attractive due to the large number of sheer molecules and signal you can generate...

...you are interested in viewing which has drawn a large crowd, making it difficult to see the actual poster...posters you are interested in viewing. For most larger conferences, poster titles and abstracts are available...

...and actual molecular size – it is possible that a large or charged FP fusion can change the subcellular ...

...gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch...

.... Picking receptors The receptor you choose is largely dependent on what cellular response you aim to ...

...groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking...address this issue, but it still points to a far larger and more complex problem endemic to biomedical ...

...Permeabilization What is the point?  Antibodies are large proteins that need a little help crossing cell membranes...