... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR.... Caulobacter crescentus is a popular model organism for studying the bacterial cell cycle, asymmetric...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...PubMed Central PMCID: PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage...CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning...

...the supplement of this paper), help each person organize his or her thoughts to facilitate honest conversation...for their time in the lab and their next steps. Addgene would love to hear about the steps taken in your...

...reagent type, target species, target name, host organism, isotype, clone details, vendor, method, tissue.../10.1073/pnas.2018488117 More resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right Isotype...

... OR enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration...interaction, thereby changing the FP’s structural organization and activating the fluorescent signal. Blue ...fluorescent molecule   Additional Resources on the Addgene Blog Check out the Rosella Autophagy Sensor Read...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...extracellular molecule? By fusing a biosensor to an organelle-specific targeting signal or a transmembrane domain...CRISPRainbow for Genome Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids...

...perfect job.   Resources at Addgene Read Additional Career Posts on the Addgene Blog Browse Our Careers ...Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies...applied anyway. I later found out the job was with Addgene. I had used plasmids from this company before and...also get the chance to contribute content to the Addgene’s Website and travel to meet scientists, making ...develop my own projects, but working remotely (Addgene’s main offices are in Cambridge MA, while I’m based...Careers Page to See What Positions Are Open at Addgene Learn How to Choose a Good Mentor for Your Professional...

...deposited these plasmids at Addgene. While deposits from for profit organizations are less common, they can...This year, Addgene welcomes three new members to the Board of Directors: Khandan Baradaran, Frank Stegmeier...assay technologies makes her a perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics... the research funded,” she says. Baradaran met Addgene Executive Director Joanne Kamens several years ...through this connection that she learned more about Addgene. “The mission to share critical reagents such as...Scientific Officer at KSQ Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically...finds the value in the wealth of resources on Addgene’s website and blog important. “The experience from...

...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition to the poster session was a first...happens): Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges...Simon Singh's "The Code Book" for this month's Addgene Book Club. I read most of the book on my way to...from those who expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious...this meeting. And of course, I want to thank the organizers of this meeting who have given the attendees ...increasingly important field.  Interested in Addgene's Keystone Poster?  We'll be sharing the poster...

... biology tools.  Addgene Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors...you keep your virtual molecular biology toolbox organized, today’s post features a list of free online molecular.../ protein database search and analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs...restriction enzyme cut sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled...available sources, as well as those deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences...tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence manipulation... Rachel Leeson.  Additional resources on the Addgene blog: Learn how the CRISPR software matchmaker...

...guest writers. Your contributions make Addgene and the Addgene Blog useful resources for the biological...It’s nearing the end of #DepositorWeek at Addgene and we’d like to show our gratitude on the blog by ...highlighting some of the great posts written by Addgene depositors. Below you’ll find brief summaries of...depositors. Interested in writing your own post for the Addgene Blog? Fill out our guest blogger form to get started... section of DNA into the genome of your target organism. In this post, Chris Richardson, a postdoctoral.... Note: Casilio is no longer available through Addgene.  Thanks again to all of our fantastic depositors... addgene...

... rigorous research plans. However, amidst the organized chaos of lab research and PhD studies, there lies...and execution plans.    More resources on the Addgene blog Experimenting with New Careers While in Grad...

... If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty ...peering right through living animals to see their organs – a liver or brain, say, or maybe a tumor. They...for optical labeling and tracking of proteins, organelles, and living cells in a spatiotemporal manner,...  Kendall Morgan...

...FAQs, Le Cong worked with Addgene Senior Scientist, Matt Ferenc, to organize the CRISPR forum's most popular...full list of 16 FAQs answered by Le Cong Read Addgene's CRISPR guide for background information on CRISPR...the most frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools: List of...scientific community and sharing these resources with Addgene. Thank you, Le!    ...

... What is an Antibody? Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...cells or tissue slices mounted to a slide, or whole organs. Because the tissue architecture is preserved, ...from the bloodstream. Then, via a blood draw or organ harvest, the labeled cells can be analyzed using...different research applications they can be used for. Addgene’s Antibody Plasmid Collection includes plasmids ...10.3389/fcimb.2013.00072 Additional resources on the Addgene blog: Read about another reagent sharing platform...

... about the role these factors played within an organism.  So how did plasmids get their name? In 1952...diversely applied to many different experiments. Addgene was founded in order to store, QC, curate, and ...cloning and gene expression. Just released: Addgene's Plasmids 101 (2nd Ed.) eBook And now for some ..... You can now download the newest version of Addgene's Plasmids 101 eBook! In keeping with our goal of...

...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...Medical Art This site has 3,000 free medical images organized into four main categories: anatomy and the human...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...

...skills guide Resources on Addgene.org Learn more about Careers at Addgene Watch out career videos   ...skilled at presenting and answering questions. Organizing a thoughtful engaging talk takes significant ...successful writers about their strategies for organizing and self-critiquing their own writings. 8. Learn...outdoor photographer.  Additional Resources on the Addgene Blog Find Tips for getting a faculty position ...

...Searching, Reading, & Organizing Write for the Addgene Blog Resources on Addgene.org Check out Our Revamped...100percentSDS.     Additional Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific...

... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...that mythical place where antibodies are well-organized, documented, and easy to find (though we might... References and Resources More resources on Addgene’s blog Antibodies 101: Monoclonal Antibodies Antibodies...

...study single cell dynamics in model organisms Resources on Addgene.org Visit our stem cell page Find plasmids...CellTag, you can find the CellTag libraries at Addgene. The Morris Lab's website also has several useful...bioRxiv (2019): 335547. Additional resources on the Addgene blog Learn more about induced pluripotent stems...