...namely that they can cause cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular... are, in most cases, interested in transferring genes to mammalian cells (i.e. transferring DNA), yet ... is (thankfully) gutted of many essential viral genes. How it is, exactly, that heaps of viral vector ...the only component that is not dependent on viral genes. It is derived from the host/producer cell membrane...full “life cycle” of expression of selfish, lazy genes of a non-living viral particle, and how we as dedicated... N. (1971). Noninfectious ARK mouse embryo cell lines in which each cell has the capacity to be activated...

...changing guidelines and regulations impacting grant management, we track grant opportunity deadlines, and ...organization to evaluate the organization’s grant readiness, identify grant opportunities, secure grant awards...schools, healthcare facilities and with private businesses. How do you get the expertise needed to enter...such as research, collaboration across multiple disciplines, writing, project management, and program evaluation...process or the whole process, including grant readiness, funding opportunity research, application writing...

...job it is to disagree with the group. They are the ones who ask for rationales behind ideas and decisions...fill out the form at any time and a member of our Business Development team would review it. This survey ...frequently observe in a scientific setting.  Powerlessness It’s hard to be creative when you don’t believe...ideas obviously worth pursuing, evaluate the “bad” ones. Bad ideas are easier to work with than nothing ... mistakes that kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https...exercise to spur innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https...

... transition Base change between purines (A ↔ G) or between pyrimidines (C ↔ T).   Base transversion ... ABE would allow it to act on newly-deaminated inosines. Pro tip! The nucleoside inosine consists of the...this promising start, the team used rational mutagenesis to produce the adenine transversion editor AYBEv3...and ABEs, they could be useful for saturation mutagenesis, and they provide a strong proof of concept for...empty space. Over several rounds of rational mutagenesis, the team progressed to gGBEv6.3, which was capable...

...activators and repressors to target genes. Repressing target genes using dCas9-based repressors Early ...repression or knockdown allow you to study essential genes whose knockout would cause cell lethality. The Krüppel...purpose. dCas9-KRAB plasmids for repressing target genes in a variety of species and cell types can be found...found on Addgene’s website. Activating target genes using dCas9-based activators The simplest of the dCas9...transcriptional activator. Plasmids for activating target genes in a variety of species and cell types can be found...

...engineering pipelines, we selected Nextflow, a workflow management system for scientific pipelines, including... existed who had created a shared resource of pipelines for genomic data. Thanks to the flexibility inherent...separate Nextflow process. In Nextflow, a process defines which command or script will run and can be thought... connect each subsequent process to the existing ones. Once our first process was working as expected,...check, we repeated our strategy and copied a few lines of our existing script into the section of the next...

... the speaker and offer your business card (yes, you should have a business card; it’s a bit more informal...smaller talks within the bigger conference, especially ones that are more career-focused. After these talks,... will probably be willing to accept your CV or business card. Be warned, though – someone accepting your... formally apply for the position. This post on Business Insider nicely summarizes good job fair practice... Reading and Outside Resources http://www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014...

...that recognize codons found in highly expressed genes (Emilsson and Kurland, 1990). Controlling gene expression...changing conditions - Organisms often need to express genes at different levels under different conditions. ...specific tRNA pools. For example, tRNAs used in genes encoding amino acid biosynthetic enzymes may be ... the problem of codon choice is to resynthesize genes in such a way that their codons are more appropriate...properly. You may be able to avoid getting your genes of interest codon optimized by ordering plasmids...use the same expression system for many different genes without having to create new constructs. However...

...in mouse embryos to generate gene modified mouse lines. Hopefully this post will help get your in vivo ...that later). Find Validated gRNAs to Target Mouse Genes To begin the in vitro screening process, you should... at the same time, and choose the most effective ones to increase your chances of gene modification in...a rapid acceleration in the production of mouse lines that could be used to study a wide variety of phenomena... et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes....generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering." Cell...

...Commercial Plasmid Prep Kit Amount 1000 Lanes 1000 Lanes 1000 Lanes Cost $200-$500  <$10 $20-30 Cost...commercial ladders cost between $200-$500 for 1000 lanes worth of ladder. The Penn State ladders are significantly...prep protocol, it cost less than $10 to make 1000 lanes worth of the 100 bp or the 1 kb ladder. Using a ...

...I really brought to the table was empathy, a willingness to listen, and an ability to remember the right...amount of conflict is actually critical in the business world. Companies and organizations that avoid ...involved, and also for the labs, organizations, or businesses as a whole. When I’m attempting to resolve a ... actively runs away from it. That can mean a willingness to make yourself temporarily uncomfortable for...while giving people the tools to resolve necessary ones on their own is even better. The Thomas Kilmann ...conflict resolution based on various levels of assertiveness and cooperation: avoiding, competing, accommodating...

...productive discussion first defines the material resources needed, then determines how much those resources...possible, this even includes clocks, timers, and phones.  As for reagents and consumables (such as pipet...time to decide what resources to keep, and which ones to abandon. It’s critical to make these decisions...1. First make decisions based on experimental timelines and specific aims. 2. Then make decisions based...productive and manageable laboratory environments. His business skills enhance his passion for science. Follow...

...526) that (1) enables efficient knockout (KO) of genes in immune cells; (2) is not immunogenic, a necessity... optimize conditions for a screen that assessed genes that regulate antigen-specific CD8+ T cell responses...screen, we used the pRDA_526 vector to knock out 30 genes putatively involved in CD8+ T cell responses to ... Ptpn2, Pdcd1, and Zc3h12a, demonstrating the robustness of the system and utility of the pRDA_526 vector...introduction of the model antigen, Ovalbumin, into cell lines to study antigen-specific responses. Find FITS plasmids...

...transcriptional effectors (LITEs) designed to bind specific genes and turn them on or off, literally at the flip of...are able to react and learn. All of this requires genes to be regulated dynamically.” To understand how ... tools to manipulate the expression of specific genes in the brain in reversible fashion. And that’s exactly... of living mice both quickly and significantly. Genes under LITE control showed a rise in transcription...researchers applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic ...

...focused on choosing a lab, but almost all of these guidelines can apply to any workplace or job. It amazes ...will need is about you. It’s worth taking time to honestly evaluate your own style of work and communication...below, the first question to ask yourself is “which ones matter to me?”  and hone in on the factors that ...Overwork – including undue pressure, impossible deadlines, unnecessary disruptions Destabilization – including...author order PI lets lab members fight it out PI intervenes if there are interpersonal conflicts PI has no...can be very helpful in uncovering strengths and weaknesses of the options. Use this blog as a guide. Start...

...and many plant scientists want to transfer large genes, smaller binary vectors with a larger cloning capacity...than that from the commonly used 35S promoter. Transgenes are also efficiently inherited in both transgenic...expression. Sahoo et al. have tested the system with genes up to 4 kb in length and confirmed successful protein... assays as well as stable expression of foreign genes in plants." PLoS one 9.6 (2014): e98988. Pubmed ...Plant Synthetic Biology Quick, Versatile Plant Transgenesis with GreenGate Plasmids Interview: Nicola Patron...

...dictator or laissez-faire; theory or practice; business-like or artistic; tech, anti-tech or pragmatic...employers love PhD students to have: working to deadlines, writing up clear and attractive reports, being...students and postdocs go on to work in patent law, business development, teaching, etc. Church also encourages...science and engineering (STEM) expertise in law, business, journalism, entertainment, politics, K-12, etc... passion, hard work, collaboration, and staying honest with personal goals. Stay tuned for the follow ...

...naturally occurring genes for cloning purposes (antibiotic resistance genes, toxin genes, and the sacB gene...gene of interest, but others will uptake plasmid backbones that re-ligated back on themselves. Therefore,...Plasmid cloning: Creativity with naturally occurring genes  At this point in the blog post, you may have realized...

...explosion SUperNova," SunTag can help you turn up the brightness in your fluorescent imaging experiments. Fluorescent...managed to track run lengths of the motor protein kinesin across microtubules. Tanenbaum et al. then tested...to traditional fluorescent proteins Improved brightness and signal-to-noise ratio Less chance of phototoxicity...used to improve CRISPR-based activation of target genes, but we’ll save that application for another day...on Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find ...

...both introduce genes into an organism’s genome (see figure) and to disrupt endogenous genes at the site ...by transposition assays of antibiotic-resistance genes conducted in HeLa cells). In their Nature Genetics... the rapid generation of stable transgenic cell lines which can robustly express your gene-of-interest...transposons rapidly generate stable transgenic cell lines. Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821...