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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 515 results for: gan

Showing: 201 - 220 of 515 results

  1. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ... editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms...obligate thermophiles without the need to grow the organisms at lower than optimal temperatures. GeoCas9’s ...

  2. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...understand what metrics would be useful and how to organize the metrics in a digestible manner.  At first,...passed, and I participated in the discussions, I began to note trends in the data, and from that, suggest...

  3. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...

  4. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...skills”. Leadership is prized at all levels of an organization and is also one of the most common criteria ...work alone much of the time, depending on the organization they lead. Effective leaders rarely give orders...

  5. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...valued This often stems from being included in organizational decision-making. It is imperative that employees...understand the part they play in the goals of the organization and feel the importance of their contributions...

  6. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...your expression system or environment. The host organism will determine the type of vector that you will...your plasmid has been incorporated into the host organism, usually achieved with the proper selection marker...

  7. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...where they are. 8)   Look into training from an organization such as COMPASS. Although COMPASS has a special... Kendall Morgan...

  10. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...possible Tell them about the Golden Stopwatch! Any organization that deposits with and/or requests materials...to all of the winners so far! Don’t see your organization below? Don’t worry--we check periodically for...

  11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ... 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can help you restrict....2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’s Guide to the Inverted Terminal...

  13. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral...

  14. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ... about the role these factors played within an organism.  So how did plasmids get their name? In 1952...terminology held until the 1960s when scientists began to study other extrachromosomal particles, particularly...

  15. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...Girls) Communication to diverse audiences Organization Mentorship Leadership Science writing...Communication Business models Conference or club organizer Management Leadership Communication ...

  16. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...that selection of a fluorescent probe (be it an organic dye or FP) is one of the most important steps in...tools. Years ago, Kurt Thorn (UCSF at the time) began an effort to collate some of the current “best” ...

  17. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...cleavage. Secondly, fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence...dynamics of apoptosis both in vitro and in living organisms to better study and understand these diseases ...

  18. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...tools by increasing gRNA stability. First, they began with the Broccoli aptamer system to visualize the... will recruit the HaloTag with red fluorescent ligands to the loci of interest. Dual color detection In...

  19. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional...mechanisms to maintain and grow these critical organizations should be a fundamental part of the scientific...

  20. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...gestures are memorable! Attend events for one organization or go to the same conferences/meetings over ...
Showing: 201 - 220 of 515 results