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  3. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium...

  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 gene, a naturally occurring animal...

  5. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...experiment today! Get ready to identify knock-in clones by reading up on validating genome edits to find...

  6. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... years of focusing on my technical skills with cloning and lentivirus production, I didn’t really start...

  7. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... size, more durable, and usually much easier to clone, express, and purify compared to antibodies. Nanobodies...

  10. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...

  12. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...SELEX). After 4-6 rounds of SELEX, the RNA pool was cloned into bacterial expression vectors to create a library...

  14. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ... you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution...

  16. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

  17. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ... own subclone, they were able to develop a 12-plex multiplex imaging system. Mixing the subclones within...their work with the “signalome,” a 12-plex mixed-subclone system using mStrawberry, localization peptides...

  18. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ...

  19. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...antigen to myeloma cells. The hybridomas are then subcloned by limiting dilution, which produces an immortalized...
Showing: 201 - 219 of 219 results