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  1. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...each of these enzymes allow you to detect enzyme activity (and therefore your antibody) either through a... antibody will have been verified to perform the same as its unconjugated version, but if you find yourself...
  2. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ... include tetracycline or doxycycline mediated activation of Cre to either induce or repress Cre expression...repair is a random process, zygotes edited using the same nuclease may not result in genetically identical...
  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...Beyond simply monitoring protein localization and activity, CRISPR tagging systems can be modified to assay... 2-3). Since donor plasmids of the same type will produce the same unique SapI 5’ overhang, different ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...
  4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ... Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts  ...Beatty et al., 2012). These features make AdV an attractive candidate for in vivo delivery of advanced genome...achieved by isogenic AdVs encoding TALENs targeting the same chromosomal region. The CRISPR/Cas9-derived RNA-...in a second paper published in Nature Methods the same year that delivering RNA-guided nucleases or TALENs...
  5. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...proteins that target specific tissues are areas of active study (for a review click here). Alternative envelopes...often: Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral ...both lentiviruses and gamma-retroviruses use the same genes for packaging (that's gag, pol, and env), ...initial infection. SIN is shorthand for self-inactivating, which is achieved by deleting a large portion...
  6. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus. Virology...instructions in the same producer cells as the virus. Because it is made at the same time as the other ... a retroviral particle, which is essentially the same for viruses and viral vectors. Key to the wild-type...
  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... one’s work. As a community, we must therefore actively work to remove the stigma around sharing negative...well for a neighboring cell type, even within the same brain region or nucleus. Just optimizing AAV expression...vectors, many from people trying to answer the exact same question I was: What AAV vector is best for my specific...wanted to see more negative data (Figure 2.) At the same time, when scientists who support open science, ...
  8. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...one area at a time and try to say “no” to other activities. Take care of yourself. Take a vacation alone...whatever works for you, but most days go home at the same time. Use time management tools and tactics to plan...need to match up exactly when you fold them. The same with sheets – wash them and put them right back ...family by forming a social circle of people in the same situation. Go to church, join a club, meet the neighbors...
  9. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... reciprocal, forming a neuronal loop which is activated and strengthened during memory formation and memory...connections that are functionally relevant, i.e. activity-dependent neuronal tracing. Apart from mapping...al., 2008) AAV6 (Towne et al., 2010), AAV8, -9 (Masamizu et al., 2011); AAV5 (Aschauer et al., 2013). Anterograde...PMCID: PMC5529245. Choi, V.W., McCarty, D.M., and Samulski, R.J. (2005). AAV Hybrid Serotypes: Improved Vectors...PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R. J., and Tuszynski, M. H. (2008). Efficient...PMID: 25374356. PubMed Central PMCID: PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi, H., Yuasa...PubMed Central PMCID: PMC5572074. Salegio, E.A., Samaranch, L., Kells, A.P., Mittermeyer, G., San Sebastian...
  10. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...host chromosomes, further minimizing potential activation of innate immunity. AAV vectors are also capable... PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in vision with...
  11. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...system incorporates the Tet-off transcriptional activation system to drive high levels of expression of ... 10 cm2 dishes. While this option is attractive because the same procedure for a single 10 cm2 dish can...systems such that it no longer requires the viral transactivator, Tat. For more information on the different...
  12. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...State University Use Nanobodies to Control Protein Activity Learn more about protein tags Read about E. coli...figures 4A-C). And since they have limited cross-reactivity between different IgG subclasses and different...primary antibody-nanobody mix can be added to your sample, thus eliminating the secondary antibody incubation...568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color staining workflow...
  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...back, better and safer. It is now one of the most active and exciting areas of science. This is hopefully...retinal cells - AAV - were becoming available. At the same time advances in human genetics led to the identification...24664759. PubMed Central PMCID: PMC4267879. 10. Samulski RJ, Muzyczka N., 2014. AAV-Mediated Gene Therapy...
  14. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...used to measure conformational changes, cleavage activity using FRET-based biosensors, and interactions ...What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent physical...341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer (FRET...
  15. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...the nucleus where they exert their recombinase activity (Logie and Stewart 1995; Metzger et al. 1995)....reintroduction, inversion and re-inversion happen at the same probability. Continuous inversion of the DNA sequence...recombination can only occur between target sites of the same sequence in FLEx vectors, the DNA sequence that ...manipulations in vivo, FLEx vectors expressing the light activatable ion channel under the control of Cre recombinase...neuron and its synapse should be visualized at the same time from two different plasmids this approach becomes...connectivity before and after the gene knockout in the same mouse.   Scientists always strive for more....
  16. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... dendritic cell function and increased B cell activation. PLOS ONE, 12, e0180664. https://doi.org/10.1371... are represented as a series of dot plots in the same order. However, in an actual flow cytometry run,...parallel.  Let’s imagine we’re looking for cells in a sample that are negative for marker A and positive for... proxy for nucleic complexity of the cell.  The sample will have been labeled using a panel of markers... dots. In a contour plot, each line contains the same number of events. Thus, contour lines that are close...
  17. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...synthesized by electronically recording fluorescence activity as fluorescently labeled nucleotides are incorporated...membrane for analysis through hybridization of a radioactively or fluorescently labeled probe. If the DNA is...searching for), then the probe will bind to the sample thereby labeling it. The label can then be visualized...visualized by autoradiography and X-ray film for radioactively labeled probes or by the presence of fluorescence...strength of the signal in comparison to a control sample. Southern blotting applications range from confirming... MstII and southern blotting are conducted on a sample from an individual without sickle cell anemia, ... largely replaced by real-time PCR to answer the same experimental questions. Real-time PCR detects and...
  18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...gate to focus your analysis on what’s biologically active. After that, the next gates fully depend on your...When using flow cytometry to analyze your samples, it is necessary to set up a sequence of gates to be...the area under the resulting curve. By default, a sample on the flow cytometer (before you set the first...Practical example: lymphocytes in a mouse tumor sample You made it! So much about the theoretical background...gated out my lymphocyte population from a tumor sample. Many cell types can be found in the tumor microenvironment...proportion between H and A (or W and A) within the same type of scatter. Here I employed an FSC-H / FSC-...pattern of positive vs. negative signal remains the same (Figure 5).   A.                                                                                                        ...
Showing: 201 - 220 of 283 results