We narrowed to 458 results for: invs
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TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....approaches. Nanoblades can also be used to non-invasively gene edit the liver of adult mice. When mice ...generating transgenic mice strains and for non-invasive in vivo gene editing. The bottom line on Nanoblades...
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Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....where and when certain genes are expressed to investigate their role in a specific behavior. This blog ...a defined brain region that is suspected to be involved in a certain type of behavior (for a review see... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog PostPublishedAug. 6, 2019, 12:55 p.m....you want combined in your offspring. Genotyping involves getting a small piece of tissue from each mouse...effectively breeding your mice. Because their generation involves the integration of a novel gene randomly into ... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....answer questions about the evolution of the genes involved in methionine synthesis (5). The budding yeast...and instructor resources is available at our Investigations in Molecular Cell Biology Website. Many thanks... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....depositing scientist, you will have the most involvement with Step 1, and, while data entry can be a chore...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human primate (NHP...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library... -
6 Tips for Grant Writing
TypeBlog PostPublishedOct. 18, 2016, 2:30 p.m....intend to be productive with the granting agency’s investment. 6. Failure is part of the process, keep your...applications there’s going to be an element of luck involved so it’s important not to throw in the towel if... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying... viral preps available! We are expanding our inventory of ready to use viral preps from the Deisseroth... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m.... of outcomes including excision or insertion, inversion, translocation or cassette exchange. By utilizing... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....seven institutions involved in the effort. This inclusive and open approach by all involved definitely contributed... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m.... factor What is fun for the audience? Getting involved and feeling "closer" to the panel. Your audience...incorporate them into the program. I have to admit to inviting interesting friends and colleagues to events for... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....well, and they thought we could be taught. They invested in us this summer – $30,000 for research and another...flower that changes color, but the experience is invaluable. This opportunity has given us the chance to ... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....into multiple bacterial species including several involved in human disease. However, the rate of transfer...for the study of essential genes such as those involved in antibiotic resistance. Are you ready to move... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...Wouldn’t it be nice if after all the hard work involved with cloning, you would at least be guaranteed... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....many many times in a short time period. PCR was invented in the early 1980s by Kary B. Mullis who later...to design PCR primers. Types of PCR Since the invention of PCR, different PCR methods have been developed... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....that change single base pairs, to chromosomal inversions and large deletions. Many of these methods rely...However, there is the chance of a chromosomal inversion, or just random mutations if the chromosome is... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....recommend using primers targeting the viral genome or inverted terminal repeats (ITR). You should always have...quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human Gene Therapy ... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....autoclaving, which also takes time. Rather than investing all of this effort in preparing reagents, I choose...quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human gene therapy ... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m....efficiency and accuracy. Golden Gate assembly involves the simultaneous activities of a Type IIS restriction...Assembly All Golden Gate assemblies feature an inverse proportionality between the complexity of the assembly... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....localization, dynamics, and behavior of a POI. As an investigator, you want a fusion tag to be inert, to not produce...has gone into making FPs monomeric, yet many investigators remain ignorant of FP dimerization. Equally ...