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Showing: 181 - 200 of 442 results

  1. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... of outcomes including excision or insertion, inversion, translocation or cassette exchange. By utilizing... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ...

  2. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human primate (NHP...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...

  4. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ... factor What is fun for the audience? Getting involved and feeling "closer" to the panel. Your audience...incorporate them into the program. I have to admit to inviting interesting friends and colleagues to events for...

  5. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...well, and they thought we could be taught. They invested in us this summer – $30,000 for research and another...flower that changes color, but the experience is invaluable. This opportunity has given us the chance to ...

  6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...into multiple bacterial species including several involved in human disease. However, the rate of transfer...for the study of essential genes such as those involved in antibiotic resistance. Are you ready to move...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...autoclaving, which also takes time. Rather than investing all of this effort in preparing reagents, I choose...quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human gene therapy ...

  9. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...Wouldn’t it be nice if after all the hard work involved with cloning, you would at least be guaranteed...

  10. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...many many times in a short time period. PCR was invented in the early 1980s by Kary B. Mullis who later...to design PCR primers.  Types of PCR Since the invention of PCR, different PCR methods have been developed...

  11. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...recommend using primers targeting the viral genome or inverted terminal repeats (ITR). You should always have...quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human Gene Therapy ...

  13. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... using droplet digital PCR Droplet digital PCR involves partitioning a PCR reaction mixture into approximately... Witte, Anna Kristina, et al. "A systematic investigation of parameters influencing droplet rain in the...

  14. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Oct. 24, 2019, 1:26 p.m.
    ...been identified in humans and catalogued in the ClinVar database. Previously developed genome editing methods...their paper, the Liu lab points out the need to investigate off-target prime editing in a genome-wide manner...

  15. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...ownership of their work and become more actively involved in the learning process. As trainees become more... and effort and/or perceived professional risk involved in setting up a CURE, especially if it’s a new...

  16. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ... placing orders per day across regions. Our investigation of model fit revealed some potential instabilities...University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...

  17. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ... in using the 3rd-generation system is that it involves transfecting four different plasmids into the ...quickly check that a construct is functional before investing time and resources into making a stable cell line...

  18. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...culture shock? Working in an academic research lab involves a few key features that can be difficult to replicate...sometimes wish for a stretch of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch...

  20. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...gene commonly associated with CRISPR arrays and involved in spacer acquisition following infection, as ... we appreciate the opportunity to share these invaluable reagents with the scientific community. Note...
Showing: 181 - 200 of 442 results