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Showing: 181 - 200 of 219 results

  2. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While...

  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...software and synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the ...

  9. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ... additionally provides detailed information on cloning procedures used to construct the deposited plasmids...

  10. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...

  12. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ... are typically knocked-in via gene targeting or cloned in-frame to the protein of interest for plasmid-based...

  13. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...use in western blots, though the original parent clone (M2) has been widely used in a variety of applications...

  14. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids...
Showing: 181 - 200 of 219 results