Optimizing Donor DNA for Enhanced CRISPR Genome Editing
Type
Blog Post
Published
March 24, 2016, 2:30 p.m.
...presumptive mechanism of action are presented in Figure 2.
dCas9 enables sequence replacement without cutting...Nature biotechnology (2016). PubMed PMID: 26789497.
2. Lin, Steven, et al. "Enhanced homology-directed human... between Cas9 (which causes the breaks) and host cell DNA repair factors (which repair those breaks) makes...example, correcting the allele that causes sickle cell anemia. However, HDR occurs much less frequently...a structure could drive sequence replacement in cells. Targeting three dCas9 molecules to a precisely ...functional Cas9 protein and the targeting guide RNA into cells by electroporation. Results from our lab and others...Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins...