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Showing: 181 - 200 of 506 results

  1. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...structure is strikingly similar to the design model at atomic resolution” (Kuhlman and Dantas et al., 2003). ...). Design of a Novel Globular Protein Fold with Atomic-Level Accuracy. Science, 302(5649), 1364–1368. ...

  2. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...Cell demonstrate how PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise and endurance...altogether. Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors...

  5. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...customizable DNA-binding domains based on transcription-activator-like effectors (TALEs) as anchors for genome engineering...and modular,” Konermann said. “Transcriptional activators are easy to expand or combine with different ...

  6. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ... bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University of Science and ...Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science...

  7. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...researchers have plans to expand the library to include activators for more cancer-related pathways and more nodes...

  8. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...

  10. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...

  14. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...

  15. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...line is known to be genetically manipulated, its anatomical origin, if cell line has ever been the target...informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to access Cellosaurus...

  16. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases Rylan Larsen New serotype...multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New serotype ...

  17. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

  18. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...

  20. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...
Showing: 181 - 200 of 506 results