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  1. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ..., and Martin L. Yarmush. "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent...., et al. "Expansion and fibronectin-enhanced retroviral transduction of primary human T lymphocytes for...

  2. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...depending on the cell line so old cells should be retired! Conversely, cells younger than the 3rd – 4th passage...

  3. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...scientists have taken advantage of the natural secretion of biochemicals and proteins through plant roots... Tagging recombinant proteins with a naturally secreted plant peptide allows for easier access and purification...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...control of biological systems (Wheeler et al., 2016; Duret et al., 2019). But the field has sometimes seen ...plasmid here!   References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...fluorescence falls within the linear window for the theoretical timer shown in Figure 1B, you can use the equation...differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells." Nature communications 7 (2016...

  6. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...activators or repressors. These fusion proteins retain the ability to recognize and bind to DNA; when ...occasions truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make edits (AC, ...

  7. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

  8. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...standard curve, and most results can be confidently interpreted as integers. Since each sample is partitioned...method you choose, resist the temptation to over interpret your results: Sybr cannot be used to determine...

  9. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...much easier. So you could imagine engineering the entirety of a cell. That's a big long-term technical challenge... What's incredible is the bioeconomy is already pretty substantial. It's estimated that genetic engineering...

  10. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ... bathroom mirror pep talk, forgotten and then retrieved your lucky socks, persevered through a nerve wracking... snapshot of the skills that you can bring into pretty much any other workplace. To get your mind working...

  11. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...

  12. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ... (Kyndt et al., 2015)! It sounds simple, and it pretty much is, but there are some tips and tricks you...having an analyzable transgenic in hand, so it is pretty important to get this process right the first time...

  13. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...within the target window, possibly due to longer stretches of duplexed RNA. Using an ADAR2DD catalytic mutant...perform C-to-U edits, the authors note that it retains its adenine deaminase ability. The authors then...

  14. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...appear reduced in ExM, which could impact how you interpret a target’s structure. For example, cytoskeletal..., Chen F, Piatkevich KD, et al (2016) Protein-retention expansion microscopy of cells and tissues labeled...

  16. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... great idea to most people in 2005, but XKCD is pretty successful!  What resources do you have?  No matter...for children” or may need text for audience interpretation. Infographics Low Combines both words and...

  17. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ... mechanism to culture only bacteria which have retained a functional copy of your plasmid of interest,... plates due to the fact that beta-lactamase is excreted. Satellite colonies are small colonies of bacteria...

  18. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...proteins were successfully expressed as fusions that retain their individual activities (1). Researchers also...DBD) and transcriptional activation (TA) domains retain their individual activities when separated (2),...

  19. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...grow. As the bacteria divide, they replicate and retain the plasmid. This allows us to make unlimited copies...deposit. Using barcoded tubes is part of Addgene’s secret to success. It helps us quickly process samples...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...antibodies. In contrast, with their many epitopes, smFPs retain their ability to be detected after sample preparation...the Addgene Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus ...
Showing: 181 - 200 of 471 results