...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which...expression. Experimental output is measured and quantified. Created with BioRender.com.   Optogenetic...optogenetics or biosensors in one system. There can be substantial overlap in the activation curves (Marshel et ...

...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral... method detects the expression of viral capsid antigen p24, the FACS dosing method correlates viral titre...reverse transcriptase activity assay uses qPCR to quantify viral RNA. All of these methods have been described...

...detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for...of NLuc has caught the attention of researchers wanting to perform whole-animal imaging, however NLuc’s...

...sequences cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs... grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will...

...delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc... *These are only starting recommendations...concurrently to minimize the impact of neutralizing antibodies developed in response to the first exposure to...AAV-PHP.eB. For this reason, the Gradinaru lab anticipates that high-dose AAV-PHP.AX will be the capsid ...efficiency, and is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), ...

...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader... HPLC (proteins or other molecules) Isolate or quantify cells, proteins or other molecules that have been...Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources on Addgene.org...