...the laser power source itself. Pulse generation (TTL pulse) is a standard electronics protocol not specific...experiments we discussed last week are only half of the battle when using optogentics to answer a research question...

...recombination sites (attL-left- and attR-right-, Figure 1). Under certain conditions, the attL and attR sites...into an attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing... and a attL-entry vector. This fragment is inserted in a multiple cloning site (MCS) of an attL-containing... attP1 sites; attB2 only with attP2, attL1 only with attR1; attL2 only with attR2, and so on. Take a look...reactions. Recombination of attP and attB sites creates attL and attR sites.   Gateway vectors contain modified... clone containing the DNA of interest flanked by attL sites. As a byproduct of the reaction, the ccdB ... BP and LR reactions. The BP reaction creates an attL-flanked entry clone. The LR reaction creates an ...

...to our guest bloggers Marcelle Tuttle and Alex Chavez! Marcelle Tuttle is a medical student at Tufts ...post was contributed by guest bloggers Marcelle Tuttle and Alex Chavez, researchers at the Wyss Institute...nmeth.3473 Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand... . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan...

...mug or reusable water bottle, you’d probably bring in a mug or reusable water bottle instead of buying yourself...contributors to the sustainability problem, how can one little change make a difference? Individual changes to...yourself a big stack of plastic cups. By initiating little changes in ourselves, we can both influence others...

... discarded.  Seeding Labs does an amazing job shuttling unwanted lab supplies to places that really need...one point I started to become very aware of how little care I took with my discards and to be mindful ...the lab materials I was using.  I started to do little things to save reagents and money.  This was the... for smaller and smaller gel apparatus until I settled on using glass slides. With just the right amount...

...color throughout the day) and doing this with very little money. So when did inspiration strike us so hard...started to entertain other options. Keira had it a little better. As a Master’s student, she had less pressure...help from SynBio Axlr8r We limped along with very little traction for quite a while, and even had some serious...on one another to celebrate good news or share a bottle of wine after a rough day, and we have great partners...

...solutions they promote help motivate you to spend a little time advocating for science and getting yourself...loved - there were booths for children to learn little things about science. We need to keep encouraging... and science education. Photo cred Alina Chan. Seattle, Washington, USA 47.6062°N 122.3321°W– Justin Sherman... professionals and supporters. I'm raising some little scientists (as you can see in the picture) and ...

..., and ColE2 plasmids) as cell division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that...the accumulation of toxic intermediates or the shuttling of the intermediates into other pathways. In cases...biotechnology 36.4 (2018): 352. PubMed PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson...

...options to choose from.  Transfer Transfers are a little trickier than gels and may require multiple runs...your protein is over-transferring! If you see very little signal, but you know you loaded a good amount of...protein-based buffers, so here’s a quick tip: Keep a bottle of chemical buffer around, which works well and...stripping* Use a harsher stripping buffer Too little signal Large proteins: Incomplete transfer ...

...incorporated at a precise genomic location, with little to no room for error. It’s no surprise then that...Prolonged cell cycle arrest can lead to apoptosis in as little as 10 hours, so read up on the timing of this approach...factors were loaded onto the break, potentially shuttling repair down a different pathway (Charpentier et...

...policy, there is often discussion of “big P” and “little p” policy.  “Big P” policy is what you might typically...activities, formal lobbying, or strategy setting.  The “little p” policy is non-governmental work happening at...areas. Whether you’re interested in “big P” or “little P” policy, the possibilities are endless leading...

... which are produced from plasmids and have very little batch-to-batch variation. Confirmation of sequence...specific protein modifications, we recommend doing a little more research into gel chemistry and/or specialty...pre-wetting and has lower background than PVDF. It is a little quicker to use, with high binding capacity, though...

...translocation of the reporter. As a result, the KTR is shuttled in and out of the nucleus depending on its phosphorylation...subcellular compartments. Second, because KTRs shuttle between the nucleus and the cytoplasm, nuclear ...

...find out how we can serve them better. Here’s a little bit of what we learned. Check Out Our Microbiology...temporary tattoos on the spot with our trusty spray bottles! We also raffled off one of our highly sought-after...

...neuropsychology) and from areas in which I knew very little. Diving into subjects like risk perception, scientific... measure how individuals respond to issues of unsettled science. Understanding just how the public utilizes...

...Must be ready to change projects frequently with little notice or choice Always working on a team/in a ... structures are common Small integrated teams, little hierarchy Real diversity and acceptance can ...

...experiment, the Morris lab identified a novel gene, Mettl7a1, whose expression helped coax cells towards successful...uncharacterized putative methyltransferase. Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression...

...contradictory, owing in part to the fact that very little was known about the role these factors played within... distribute them all in the name of making it a little bit easier for scientists to conduct their research...

...reasoning,” the catchall term for a range of neat little tricks our minds perform to avoid cognitive dissonance...10.1073/pnas.1704882114 Kahan, D., Peters, E., Wittlin, M., Slovic, P., Ouellette, L. L., Braman, D., ...

... the Addgene lab. In this post, I’ll tell you a little bit about what I learned from a New England Biolabs...CosMCPrep system for this, but thought we could learn a little bit more about high throughput DNA prepping by ...