Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
Type
Blog Post
Published
Dec. 13, 2018, 1:14 p.m.
...shown that cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal...Shen, 2011). To our knowledge the effect of hyperosmolarity on AAV production yields had yet to be examined...References
1. Coroadinha, A. S., et al. "Effect of osmotic pressure on the production of retroviral vectors...Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate." Biotechnology and... and antibody production under conditions of hyperosmotic stress." Advances in animal cell biology and...., and Bernhard O. Palsson. "Effect of medium osmolarity on hybridoma growth, metabolism, and antibody...18597326.
5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth ...