...through a repository, they remain accessible during these times. Centralized reagent repositories, also known... Figure 1: Biological repositories that distribute mouse strains, plasmids, cell lines, and much more...these plasmids from Addgene. This publication then resulted in the development of a new plasmid, pCMV14...continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the rapid...to be deposited into repositories, and financial support to maintain repositories’ capacity for storing... the scientific community can support repositories. Repositories are essential for the advancement of ...

...18,359 viral items, and 166,247 plasmids. We welcomed 18,899 plasmids, deposited from 1,106 labs in 504... countries, into our repository, while expanding global access to our repository through our distributor...vectors. At 3500 plasmids, this represents the largest single deposit in our repository and was a three–year...announce that we are now able to ship rabies plasmids in our repository to international requestors.    I would...14 deposits, that became available through our repository in 2023.  In June, we announced a new partnership...September, we released the fourth edition of our Plasmids 101 eBook. This updated version of one of our ... launch our Antibody Data Hub, an open-access repository for antibody validation data. We’ll continue ...

... in one place. Plasmid mapping These tools are for viewing, editing or making plasmid maps, but can also...make plasmid maps. Free for academic users. Serial Cloner: Free desktop-based software for plasmid design...design and mapping. ApE (A plasmid Editor): A free, donation-based plasmid analysis tool including editing...Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA.   DNA / ...data repository for validation data on Addgene/CLOVER viral vectors.  Caspedia: A data repository for ...Primer design. Plasmid mapping. DNA sequence analysis. We all have our favorite tools for tackling these...The free SnapGene Viewer is great for looking at plasmid maps and viewing sequencing traces, while the paid...

...sharing of plasmids between scientists. The service would allow people to add a gene to a repository others... years to reach our first major repository milestone — 10,000 plasmids deposited and available — and nearly...the 500,000 plasmids distributed milestone! We were thrilled to see the impact our repository was having...20th anniversary and the year our repository grew to over 150,000 plasmids, shipped out our two millionth...frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan, and Benjie...meant we could grow accessibility alongside our repository.  “I’m sure you hear it all the time, but Addgene...Addgene before we were a “labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP...

...you out. What is the Open Repository of CRISPR Screens? The Open Repository of CRISPR Screens (ORCS) database...CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for your research ...This post was contributed by the Open Repository of CRISPR Screens. Imagine you’ve just discovered that...research. Fortunately, there’s a growing open repository of CRISPR screen data that might be able to help... resource developed by the Biological General Repository for Interaction Datasets (BioGRID) in order to...biologists. Figure 1: The BioGRID Open Repository of CRISPR Screens (ORCS) homepage. Screenshot...Screenshot updated 2024.   Using the Open Repository of CRISPR Screens BioGRID ORCS currently houses over ...

....  When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and in ...ready-to-use antibodies. Now, we think of ourselves as a repository for DNA-based materials, including our popular... kits and libraries. This DNA-based materials repository contains 139,868 reagents from 5,821 labs in ... system for scientists using materials in our repository, from our customer and science support to our...addgene.org! Fun fact! Did you know you can search for plasmids from a publication by pasting the paper's title...

...,000 CRISPR plasmids in our repository, and we have shared almost 300,000 CRISPR plasmids and 12,000 CRISPR... (A) Cumulative CRISPR plasmids shipped by year. (B) Cumulative CRISPR plasmids deposited with Addgene...these CRISPR plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a...once made available through the repository. And the number of plasmids and libraries deposited and requested...CRISPR plasmids requested each year by year of deposit (Figure 3), we saw that once CRISPR plasmids become... CRISPR plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age)...Total CRISPR plasmids deposited by country; square size is proportional to number of plasmids deposited ...

...curating plasmid collections. Addgene's "How to cite this plasmid" section Anytime you use a plasmid from ...variety of plasmids in the repository. And last, but certainly not least, we often feature new plasmid technologies...Special Collections Pages Suggest Plasmids for the Repository Find Plasmids for Your Research  ...process, we explain how to cite plasmids from Addgene right on our plasmid pages and provide a citation ...comparing two plasmids, we recommend reviewing the cited papers to determine whether the plasmid has been ...easier for you to browse through the many plasmids in the repository, our scientists have curated a number...collection or if you think a particular plasmid or type of plasmid is missing from our collections pages...

...maximize the use of both when making plasmids. Use repositories Repositories such as Addgene help share materials...pages to more easily find plasmids Search for a plasmid in Addgene’s repository ...cost required to make a plasmid. In 2023 (and at closer to 2 million plasmids shared than not!), we think...great Plasmid Debate of ’18, Blue Flame depositor John Doench calculated the time to make a plasmid. Doench... make a plasmid? Doench also calculated the cost of consumable reagents used to make a plasmid, which ... to make plasmids that already exist, and we significantly reduce costs of producing plasmids with high-throughput...lot of plasmids, it’s almost always faster to order from us!) Ask the PI If you want a plasmid for free...

...source data repositories Over the past decade or so a number of general purpose repositories have been ...value the repository integrations, because when RSpace documents are deposited into the repositories, links...research; and (3) connectivity to open source data repositories. To illustrate this, let’s take a look at the...data sources, tools used in research, and data repositories. Below I describe how each of these kinds of...apps, colony management software, and method repositories like protocols.io. These tools are essential...integration with protocols.io, the protocol sharing repository. As illustrated in this video the integration...one of five general purpose open source data repositories: Dataverse, Zenodo, Open Science Framework, ...

...bacteria with the plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows...Learn all about Plasmids in our Plasmids 101 series! ...Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the...about Developing Lab Management Software    The plasmid's journey begins First, each package is opened and... to hundreds of samples, with an average of 10 plasmids per deposit. Each week an average of 16 deposits... prepares the sample for entry into Addgene’s repository. For long-term storage in our facilities, every...allow us to store multiple types of tube for each plasmid we have at Addgene. Each sample at Addgene has ...

... here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids sent to happy scientists around... you all the plasmids you need. We're focused on our mission – documenting your plasmids, storing them...500,000 plasmids go? Far and wide – that's where! Check out the map below which depicts where plasmids have...Sidney Street. Then the plasmids travel to labs around the world. We've shipped plasmids to more than 90,000...scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside of the... being part of our growing community! Our plasmid repository succeeds because of our amazing community...providing quality control and customer support, making plasmids easy for you to find and request in our online...

...the 879 viral vectors in our repository are produced from only 413 plasmids containing a genetic payload...conditions. Sharing our experiences Addgene’s plasmid repository gave us the experience to launch a high-quality...been finding and sharing stories unique to our repository and resources. As editor of Addgene’s eBooks ...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...order high quality viral vectors from the Addgene repository can save researchers significant time and removes...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...

...creating a repository that made it easy to share plasmids, both for people depositing plasmids and for people... materials in our repository. We started to increase accessibility to our repository by making it easier... Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression...Expression Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design Your... Verify Your Plasmid Using a Restriction Digest Analysis Videos What is a Plasmid? How to Design...video, however, is “What is a Plasmid?”, An animated explainer of a plasmid and its parts that helps the...incremental changes. It was only after creating a plasmid repository and shipping internationally, after all, ...

...asking you for plasmid suggestions. Can’t find a plasmid you’re looking for in the repository? Let us know... with #suggestaplasmid, the name of the plasmid and a publication where we can find the plasmid. While...Oh! This plasmid would be perfect for my next experiment!” but were unable to find the plasmid at Addgene...here to help with our Suggest a Plasmid Campaign. How to suggest a plasmid The scientists at Addgene work... our "Suggest a Plasmid" form Fill out the form with the name of the specific plasmids you’d like to suggest...work hard to seek out plasmids to add to our collections pages, to comb through the literature for potential...depositing, we hope this campaign will expand the repository in ways that will be most useful to you, the ...

... dug into the data from our repository to find our most requested plasmid in 2015. In line with all of...excited to announce that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR...fantastic research tool has been requested from the repository nearly 1,000 times in 2015! If you haven’t already...put to such great use. It’s just one of the many plasmids being distributed to and accelerating research...Knockouts Read Some Common Ways to Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website...Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries ...

...deposit their plasmids to the repository. These protocols show up on the related plasmid pages and can...consistently been one of the repository’s most frequently requested plasmids. I wondered how many scientists...you’re working with plasmids and you decide to share them using Addgene’s repository, you can also share... your protocols onto your plasmid page, scientists who request your plasmid will have easy access to your...restriction digest of plasmid DNA, and bacterial transformation. And more plasmid-related educational resources...be found in our Plasmids 101 blog series. Want to learn more about sharing your plasmids via Addgene? Check... resources, such as protocols, Plasmids 101 articles on various plasmid features, and reference tables...

...is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene and we distribute...opportunity for Addgene to present our own data on plasmid deposits and distirbution for these fast moving...distribute over 130,000 plasmids in 2014. Thus, we are in a unique position to observe and quantify how...scientific community. Data on CRISPR and synBio plasmid sharing In a recent review on CRISPR technology...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...trends for Zinc Finger, TALE, CRISPR, and SynBio plasmids over time. You can see these data in the slide...molecular biology technology? Have you deposited your plasmids yet?      ...

...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different... Addgene repository. Read on to learn more.  What will be available?  Over 3000 AAV plasmids in the Addgene...Addgene repository will be available for Packaged on Request service. A small subset of AAV plasmids will...both our depositors and requestors.  Requesting Plasmids available for Packaged on Request consideration...recommend you check the original paper of your AAV plasmid of choice for more information on its performance...performance.   Titers We expect titers for plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013...

... Bank (DSHB), directed by David R. Soll, is a repository for monoclonal antibodies, distributing over ...against new cancer biomarkers. We believe that repositories, such as Addgene and the DSHB, play an important... to ensuring affordable access to materials, repositories provide standardized quality-control and technical...about Scientific Sharing: The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Data...