Special Delivery: Fluorophore Targeting for FRET Studies
Type
Blog Post
Published
July 19, 2016, 2:30 p.m.
...requirements are different for the donor and acceptor fluorophores. The donor must be delivered to an exact... when donor and acceptor are close together. The binding of acceptors >100 Å away from the donor doesn...Let’s consider the donor first where labeling specificity is most important.
Donor labeling and the need...for specificity
Donor fluorophores need to go to the right place. Mis-targeted donors never “see” FRET...by a sea of uncoupled donors.
The best method for targeting a fluorescent donor to your protein is to ...Madani et al., 2009).
Donor and acceptors: Putting it all together
The FRET donors and acceptors described...table below for donor and acceptor pairs that you can use in your FRET experiments.
Donor
Acceptor
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