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We narrowed to 86 results for: chm

Showing: 1 - 20 of 86 results

  1. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...that make each tool unique. The CRISPR Software Matchmaker is composed of these features and enables you...at the glossary section of the CRISPR Software Matchmaker, it provides a list of terms I think are important...going shopping for one. Use the CRISPR Software Matchmaker to select the best tool based on your needs. ... crispr software matchmaker...

  2. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ... you'll find in this post: MagIC-Cryo-EM for enrichment and structure determination LEA proteins protect...marking astrocytes MagIC-Cryo-EM for sample enrichment and structure determination By Mike Lacy Conventional...and Concentration (MagIC)-cryo-EM that combines enrichment and imaging of proteins captured on magnetic ...proteins ending with a GFP nanobody (Figure 1). This enrichment streamlines the purification and minimizes sample...    Figure 1: MagIC-cryo-EM for sample enrichment and structure determination. A) Schematic of ...

  3. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...and easier to use with other strains of E. coli, Schmidl et al. condensed the system to two streamlined ...gene expression at very high or very low levels. Schmidl et al. chose to optimize these systems to make ...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in ...light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014...

  4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...display to increase the enrichment of target-specific sybodies. Final enrichment via enzyme-linked immunosorbent...Ribosome display The Seeger lab starts with a “pre-enrichment” selection step using ribosome display because...sybody candidate. These tags are used in a final enrichment step for identifying sybodies that bind strongly...

  5. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ... fluorescence. Transient reporter for editing enrichment (TREE) The Wang and Brafman lab developed a transient... transient reporter for editing enrichment (TREE) to purify single base edited cells without the need ...A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9 or cleavage by ...Brafman DA (2019) A transient reporter for editing enrichment (TREE) in human cells. Nucleic Acids Research...

  6. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...doi.org/10.1021/acssynbio.0c00581   New tool for enrichment of ABE-targeted cells By Ellie Adams Single base...mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify base edited cell ...the past.   Figure 1: Identification and enrichment use of XMAS-TREE. a) Cells are transfected with...

  7. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...generated a strong streptavidin signal (~250-fold enrichment) when compared to a scrambled RNA motif control...labeling with BASU still generated a >30-fold enrichment compared to E. coli’s BirA*. Faster labeling ...-associated sequences had lower IREB2 binding enrichment compared to wild-type sequences and that loss...

  8. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...Many thanks to our guest blogger, Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium...This post was contributed by guest blogger Sarah Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory...

  9. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...virion surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence... of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking...

  14. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...., Allam, A., Kissling, L., Marquart, K. F., Schmidheini, L., Solari, C., Balázs, Z., Krauthammer, M.,... A., Tálas, A., Kissling, L., Benvenuto, E., Schmidheini, L., Schep, R., Damodharan, T., Balázs, Z., Janjuha...

  15. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...population is key. Loss of representation due to enrichment or depletion of specific gRNAs can lead to skewed...several cell types can strengthen your results. Enrichment or depletion of multiple gRNAs targeting the ...

  16. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...biology of adenoviruses (AdVs). AdVs are ubiquitous (Schmitz et al., 1983), yet rarely cause severe disease ...doi.org/10.3181/00379727-84-20714. PMID: 13134217. Schmitz, H., Wigand, R., & Heinrich, W. (1983). Worldwide...

  17. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...is used to separate the beads from the media (Brechmann et al. al., 2021) while the media is aspirated....2019.12.017. Epub 2019 Dec 28. PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri...

  18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...phosphopantetheinyltransferase) – These enzymes catalyze the covalent attachment of a fluorophore ligand to a peptide tag (Figure... the speed and selectivity of the fluorophore attachment, the brightness of the resulting conjugate, and...

  19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...called BLESS (direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing.../biot.201400046  Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin ...

  20. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...regions a chance to fold properly. For example Pechmann and Frydman found that tracts of non-optimal codons...20506237. PubMed Central PMCID: PMC2970903. 6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary ...
Showing: 1 - 20 of 86 results