...This Depositor Week, we're highlighting eight amazing posts on research done in our depositor labs. Some...written by Addgenies and some are written by the depositors themselves. All of them have cool new tools, ...scientists to use. As always, a big thank you to our depositors for sharing your science with us! Corralling ...with Tim Miles: While not technically a research story, I couldn't resist adding in this excellent Q&A ...one of our favorite posts from 2022! Wow! Our depositors do some really incredible science - I hope you've...try in your own work!) If you've got a research story you want to share on our blog, please reach out ...

...fluorescence or voltage indicators.   Viral Vectors and Voltage Indicators As previously mentioned...Voltage indicators are voltmeters for cells! In this blog we will review how these indicators work, their...as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness of fluorescence...upstream component, the chemical factor, of a naturally occurring voltage indicator. To give an example, a genetically...specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic...Resources on the Addgene blog Viral Vectors 101: Chemogenetics Viral Vectors 101: Optogenetics Fluorescent...their use as biological tools. Why Use Voltage Indicators? Electrical signals are integral to many facets...

...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many...types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral vector components of...of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for all the...NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from:...adeno-associated virus (AAV). The components of these vectors are also split across several plasmids (often three...called helper plasmids. Below, we’ll breakdown the vector components first by virus and then by function....respectively, act as RNA polymerase II promoter and terminator regions and are slightly different in sequence...

...understand this regulatory network. The transcription atlas and library The transcription factor library ...Transcription factors (TFs) act as the gate keepers for gene expression, turning transcription on and...Computational analysis was used to infer differentiation trajectories and identify both up- and down-regulated pathways...deliver multiple TFs to each cell, allowing for combinatorial overexpression programs on cellular processes...Resources  References Joung J. et al., A transcription factor atlas of directed differentiation. Cell. 2023. ...

...about her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...posts in this Mentoring for Scientists series, you have: realized the value of having a mentor, developed...finding mentors and, perhaps, asked someone to support your career development as your mentor. How do ...with a mentor.The responsibility of arranging meetings and setting the direction in a mentoring relationship...An undergraduate can mentor a high school student, a graduate student can mentor an undergraduate, a postdoc...postdoc can mentor a graduate student – you get the idea. There are many advantages to being a Mentor: Learn...want more great advice on mentoring, click below to download the "Mentoring for Scientists" ebook, which...

...opportunities: Try virtual mentoring at Mentornet.net. This is an online database of mentors you can tap for email...someone to be your mentor Mentoring is a “loaded” word.  If you ask someone to be your mentor, it may sound..., get more advice on mentoring: What Makes a Good Mentor? Choosing a Good Mentor for Scientists  ... are potential mentors all around you. This third article in the Addgene Blog Mentoring series will cover...meet, the more potential mentors you will identify. Finding potential mentors is one of the many reasons...tips for finding mentors in this recent blog 5 Strategies for Finding Your Ideal Mentor. One of the best...researcher Connie Cepko to learn about her mentoring style. Mentors at home and away We should all have a ...

...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...expressed via cells cultured with the vectors. This allows the viral vectors to reproduce only in experimentally...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...viral particle is called a virion. Finally, viral vectors, which are used in the lab, are distinct from viruses...copies itself and “splits” into two cells. Viral vectors Viruses are quite appealing as molecular biology...

...search of phages for therapy Phage Directory started out as a directory of phage researchers and the phages...can be sent out to the directory earlier, but the researchers in the directory are volunteering their ...says. As the labs in the directory step forward to help, the Phage Directory team must decide which labs...Creating a repository of phage data and information Since the inception of Phage Directory ~1 year ago...). The pair quickly teamed up to create Phage Directory in a whirlwind three days in November 2017. ...find much-needed phages for patients.   A phage directory is crucial because it's hard to find the right...a last resort, so a request for phages to the directory does not typically come out until nearly the last...

... Esq and Carl Paratore, MBA, CPA to the Addgene Board of Directors. Katz is the Director of Intellectual...School and Paratore is the General Auditor at Tufts Health Plan. The addition of Katz and Paratore onto the...Learn more about Carl Paratore, MBA, CPA Mr. Paratore serves as the General Auditor at Tufts Health Plan...presence in the biotech sector in both patent law and general life science regulatory law. My role at Suffolk...employment front. Paratore: I work at Tufts Health Plan and my role there is general auditor. I oversee a ...the Board of Directors brings complementary experience and insight to the now 10 member board at Addgene...that Addgene was looking for new board members. Paratore: Kristen Darby, who’s also a board member, reached...

...a good mentor? Where can I find a Mentor? What is this I hear about “group” or “peer” mentoring? How do... been organizing mentoring programs for scientists and doing training to help mentors and mentees have...to learn about her mentoring style. Top 7 questions scientists ask me about mentoring In this blog series... scientists ask me about mentoring. Here they are: What makes a good Mentor? How do I choose an Advisor... do I ask someone to be my Mentor? I have a Mentor – what do I do now? What are the benefits of taking...seeking out mentoring as an important aspect to their ongoing success. There were unfortunate stories from researchers...did not serve as good mentors, but many had examples of very productive mentoring relationships with advisors...

...tend to have a story structure: beginning, end; cause, effect; lesson, learning. Storytelling is a crucial...even tell short stories during weekly lab meetings. When scientists tell good stories, they engage their...appreciation for their research. Long story short, telling a good story is something that can be learned, ... your career. Story Telling at work Don’t believe me? Here’s an example of storytelling to answer an informational...conclusion. Advice for Telling a Good Story Start at the start Any good story needs a solid point to kick off...of versions of your story and find the one that fits your audience. Enjoy the story you’re telling There...collective endeavour. Your story should be too. Use drama Use any rhetorical technique you have in your...

...2022 Blue Flame Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories   Friday: Specialized...Hello and welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social...we’re certainly going to try!      Here’s our Depositor Week schedule:  Monday: Thank you post (this is...Specialized Depositor Shout-Out  We'll also be doing giveaways on Twitter, so keep an eye out there for a ...cool Addgene swag.  And, of course, to all our depositors: thank you so much!!!  (A special shout-out to...

... for a curated list of coregulators). Coregulators can function as coactivators and corepressors depending...complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex is composed...feature afforded by coactivator complexes suggests that coactivators may be integrators of multiple cell ... W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators. Cell 108, ...tissue and organ level by nuclear receptors (NRs), their coregulators, and their physiological endocrine...Nuclear receptors and their ligands NRs represent the largest family of transcription factors found in...illnesses, and the metabolic syndrome. Coregulators Coregulators constitute a group of some 400 molecules...

...searched for and eventually identified a postdoctoral mentor. A good mentor typically writes the grants that ... of reference- the graduate school mentor, the postdoctoral mentor, and at least one more person that ...was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia Research ...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting... of your postdoctoral training experience. If you're going to commit to doing a postdoctoral fellowship... you how to write as well as mentor. Yet, even the most dedicated mentor is not always available for mentees...different roles? A second or third mentor. Therefore, seek out additional mentors. I found this beneficial for...

...Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can ...Three generations of adenoviral vectors: the first generation vectors are deficient in the E1 and E3 regions... second generation vectors are deficient in E1, E2, E3, and E4. Gutless Ad vectors (helper-dependent (...creating adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene...Adenovirus type 5. Three generations of adenoviral vectors The adenovirus virion is a non-enveloped particle...medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes, AdVs cannot...lysate is your primary low titer rAdV-S, and can be stored at -80C for later use or used immediately for amplification...

...use viral vectors for many experimental purposes. To help you make sense of all the viral vector information...Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors? Check out our ...our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the Image Above ... viral vector...

... Read All of Our Editor's Choice Blog Posts Last month there was plenty of excitement at Addgene. Our...Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, gives a thorough yet concise rundown...Grad School Advice Part 2: Building Community  My editor’s choice pick for this month is “Grad School Advice... editor's choice...

... Read All of Our Editor's Choice Blog Posts As I’m sitting in the San Francisco International Airport...surpassed 60,000 views for the month of September! Historically we do better in September than in the summer...for FOP, Running for AJ I have two picks for the editor’s choice posts this month. The first, "Technique...contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at ... editor's choice...

...In response to a bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the...by PCR & Single Base Editing with CRISPR For my editor’s choice picks this month, I’m picking two posts...give a great science talk from Addgene Executive Director Joanne Kamens. I highly recommend taking a look...Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science...

...each and every month, we've decided to start an "Editor's Choice" series. Each month, I'll summarize the...on topics ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it...I love all of our blog posts, my pick for the "editor's choice" post this month is from guest blogger ... editor's choice...