CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
Type
Blog Post
Published
Oct. 6, 2020, 1:15 p.m.
...Synergistic Activation Mediator (SAM)
Description
SAM uses specially engineered sgRNAs to increase transcription...gene activation compared to other CRISPR activators, making it a popular method for gene activation experiments...gene regulation (activating multiple genes at once), however, SAM exhibits activation levels comparable...than first generation activators while showing lower activation levels than SAM. One drawback of this ...higher activation levels than the initial dCas9-VP64 activator, but lower levels compared to the SAM system...common choice for CRISPR activation.
Newer methods for CRISPR activation
While SAM, SunTag, and VPR have ...components of SAM and SunTag activators, fusing the epitope tag of SunTag with the P65-HSF activation domains...