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Showing: 1 - 20 of 285 results

  1. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... and in fact, of how adenoviral vectors work. What are adenoviruses?   Adenoviral vectors are commonly... caused by either SARS-CoV-2, Adenovirus type 41 (AdV-41), or Adenovirus-Associated Virus type 2 (AAV2...derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which ... less likely for adenoviral vectors – namely due to the removal of certain adenoviral genes that apparently...existing for that adenoviral vector (Hofmann et al., 1999).  The ability of the adenovirus particle to activate...). Ovine Adenovirus Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. ...Cellular immune response to adenoviral vector infected cells does not require de novo viral gene expression:...

  2. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ... are based on Adenovirus type 5. Three generations of adenoviral vectors The adenovirus virion is a non-enveloped...the final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references...transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page  ...Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can ... surrounding an inner core which contains the adenoviral genome. The genome itself is a linear double-...ends.   Figure 1: A) The structure of an adenovirus: the virion is ~ 100nm in size, and consists ...forming the nucleocapsid. B) Three generations of adenoviral vectors: the first generation vectors are deficient...

  3. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...out to study how protein turnover is regulated in neurons. Protein turnover is the combined effect of...visualizing protein turnover in situ in neurons. The classic approach for studying protein turnover is radioactive...whole de novo synthesized proteome. However, FUNCAT alone cannot be used to determine the turnover of one... worthy of a PhD: How do you visualize protein turnover within a neuron? For my PhD I studied a synaptic... with this method it is not possible to follow turnover of individual proteins in situ.  For the visualization...resolve the technical hurtle of monitoring protein turnover of specific proteins in situ. I was in luck when... in Frankfurt am Main, Germany. She presented a novel technology, back then still unpublished, that allowed...

  4. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ... would therefore consider myself a neuroscience novice (newb). In this post, I’d like to highlight some...some of the things that popped out to me as a novice and indicate why other neuroscience newbies might ...artificial intelligence (including a team that created a novel form of AI that can learn new tasks and beat humans...using CRISPR to generate new knockout mice, using adenoviral vectors to deliver IGF receptors to the brain...between different fields is quite vast. If you’re a novice neuroscientist looking to get into the field, this...

  6. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...pipette tips in a high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high ...We should continue to examine our behavior and innovate solutions and practices to meet higher environmental...

  9. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...including development, the immune system, and cell turnover. Apoptosis is a highly controlled process that...now (Zhang et al., 2019). Conclusion FlipGFP is a novel fluorogenic reporter that has a large dynamic range...

  10. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ..., we need to take sex into account. Gendered Innovations is an international, collaborative project—funded...creative power of sex and gender analysis for innovation and discovery. Tackling sex and gender in research...organizations. “Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology...invested in the trial). The goal of gendered innovations It is crucially important to get the research... the beginning. This is the goal of Gendered Innovations. This project: 1) develops state-of-the-art methods...of how gender analysis leads to discovery and innovation. One such case study looks at the effects of ...didn’t know why. Eventually, through a Gendered Innovations workshop in Norway, the team realized they should...

  13. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...last updated Dec 10, 2020 by Benoit Giquel.   Adenoviral vectors (AdVs) have been used for many years ...and represent one of the most studied viruses. Adenoviruses are non-enveloped double-strand DNA viruses ...targeted DNA knock-in (right) by CRISPR/Cas9. Adenoviral CRISPR vectors (left) or CRISPR vectors and donor...BioRender.   Delivering gRNA and Cas9 using adenoviral vectors As compared to other viral vectors, AdVs... breaks “that always happen naturally.” These adenoviral CRISPR/Cas9 genome editing tools developed and...mammalian cells. Applications of CRISPR delivery by adenoviral vectors Since then, other groups have successfully...switching of the relevant gRNAs and donor DNA Adenoviral CRISPR/Cas9 genome editing tools can also be ...

  14. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...experiments to the Data Hub. Submit your data by Nov. 12 to compete. The top ten participants will be ...learn more here - and remember, submissions are due Nov. 21!   ...

  15. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...vol. 5, no. 1, American Chemical Society (ACS), 4 Nov. 2015, pp. 99–103. Crossref, https://doi.org/10.1021.... 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, pp. 3393–405. Crossref, https://doi.org/10.1021.... 1, Springer Science and Business Media LLC, 13 Nov. 2017. Crossref, https://doi.org/10.1186/s12896-017...vol. 8, no. 11, American Chemical Society (ACS), 5 Nov. 2019, pp. 2593–606. Crossref, https://doi.org/10.1021...

  16. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ..., Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014...Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24;438(7067):441-2. PubMed. Find the plasmids from...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... Methods 2014 Nov 24. doi: 10.1038/nmeth.3179. Martell et al., Nat Biotechnol 2012 Nov;30(11):1143-8...17):12005-15. Hernandez et al., Autophagy 2013 Nov 1;9(11):1852-61. A quicker method for protein... will turn green. Mashiko et al., Sci Rep 2013 Nov 27; 3:3355. Rinehart lab reagents for improved...TALEN construction. Sakuma et al., Sci Rep. 2013. Nov 29;3:3379. Interested in more genome engineering...the EF1a promoter. Livet et al., Nature. 2007. Nov 1;450(7166):56-62. Cai et al., Nat Methods. 2013... report mitochondrial turnover Mitochondrial health, transport and turnover are important features...cell culture to report changes in mitochondrial turnover and transport. pMitoTimer from Yan’s lab, which...

  18. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...absorption coefficient of a protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID:...104(2):602-11. doi: 10.1002/jps.24259. Epub 2014 Nov 21. PMID: 25418950; PMCID: PMC4312221....

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 10.1128/AEM.02296-13. Epub ...303-313. doi: 10.1007/s00253-018-9485-4. Epub 2018 Nov 3. PMID: 30392122. Pfeiffer, Barret D., et al. “Refinement...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... genetic recombination of plasmids. Nature. 1981 Nov 12;294(5837):184-6. doi: 10.1038/294184a0. PMID: ...mediated by 28 bp direct repeats. Mol Biotechnol. 2008 Nov;40(3):252-60. doi: 10.1007/s12033-008-9082-3. Epub...References References Bacolla A, Wang G, Jain A, Chuzhanova NA, Cer RZ, Collins JR, Cooper DN, Bohr VA, Vasquez...
Showing: 1 - 20 of 285 results